木薯DBE和SBE基因的序列分析及其茉莉酸甲酯诱导表达特性

Sequence Analyzing of DBE and SBE Genes and Expression Characteristics Induced by Methyl Jasmonate in Manihot esculenta Crantz

为研究木薯中调控支链淀粉合成的DBE和SBE基因如何被茉莉酸信号调控,首先对木薯基因组数据库中的Me DBE和Me SBE2.1基因进行序列分析,二者都具有α淀粉酶催化结构域且启动子区都具有茉莉酸响应元件等多种响应元件。以木薯品种‘华南八号’悬浮培养细胞为材料,利用q RT-PCR检测木薯Me DBE和Me SBE2.1基因在茉莉酸甲酯处理后的表达特性。结果显示:Me DBE基因的表达在最初短暂上调后持续下调,而Me SBE2.1则持续上调后又恢复最初水平,说明Me DBE和Me SBE2.1基因可被茉莉酸信号调控,进一步推测表明,其受到不同激素信号整合后的综合调控,以影响支链淀粉的生物合成。

In order to explore how jasmine acid signals regulate the amylopectin synthesis involved genes, Me DBE and Me SBE2.1 gene sequences are extracted from cassava genome database. Sequence analysis shows that they both have typical Alpha amylase catalytic domain and a variety of response elements such as jasmine acid responsive element in promoter region.Using suspension cultured cassava ‘SC8＇ cels as material, this paper investigates the Me DBE and Me SBE2.1 by quantitative real time PCR technique after chalenged methyl jasmonate. The results showed that Me DBE gene expression is increasing firstly and then decreasing constantly, whereas Me SBE2.1 gene expression kept elevating constantly and then fals back to initial level. These data suggest that Me DBE and Me SBE2.1 genes might be regulated by jasmine acid signals, and their function on affecting the biosynthesis of amylopectin could be regulated by integrating different hormones stimuli.