摘要
为开发利用澳洲坚果青皮,采用高效液相色谱法(HPLC)研究其对羟基苯甲醇、3,4-二羟基苯甲酸、对羟基苯甲酸、对羟基苯甲醛的测定方法和含量。结果表明,澳洲坚果青皮中,4种多酚的最佳测定条件为:以1%乙酸水溶液和甲醇为流动相进行梯度洗脱(0 min,87%A;5 min,85%A;25~30 min,60%A),检测波长260 nm,流速0.8 m L/min。并测得青皮中对羟基苯甲醇、3,4-二羟基苯甲酸、对羟基苯甲酸、对羟基苯甲醛的含量分别为(1.3±0.12)、(0.19±0.001)、(0.046±0.003)、(0.15±0.00 9)mg/g。该方法简单、高效、实用。
For the development and utilization of Macadamia green husk, this paper studies the determination method and content of p-hydroxybenzyl alcohol, 3,4-dihydroxy benzoic acid, p-hydroxy benzoic acid and p-hydroxy benzldehyde by HPLC. The results showed that the best determination condition of 4 polyphenols in macadamia husk is 1% acetic acid aqueous solution and methanol as mobile phase in gradient elution(0 min, 87% A; 5 min, 85% A; 25-30 min, 60% A), detection wavelength is 260 nm and the flow rate is 0.8 m L/min. The content of p-hydroxy benzyl alcohol, 3,4-dihydroxy benzoic acid, p-hydroxy benzoic acid and p-hydroxy benzldehyde in macadamia husk is(1.3±0.12),(0.19±0.001),(0.046±0.003),(0.15±0.009)mg/g. This method is simple, efficient and practical.
出处
《热带农业科学》
2016年第7期106-111,共6页
Chinese Journal of Tropical Agriculture
基金
公益性行业(农业)科研专项(No.201303077)
广州市科技计划项目(No.2014J4500020)