高效液相色谱法测定澳洲坚果青皮中的4种酚类物质

Determination of 4 Polyphenols in Macadamia Green Husk by High Performance Liquid Chromatography

为开发利用澳洲坚果青皮,采用高效液相色谱法（HPLC）研究其对羟基苯甲醇、3,4-二羟基苯甲酸、对羟基苯甲酸、对羟基苯甲醛的测定方法和含量。结果表明,澳洲坚果青皮中,4种多酚的最佳测定条件为：以1%乙酸水溶液和甲醇为流动相进行梯度洗脱（0 min,87%A;5 min,85%A;25~30 min,60%A）,检测波长260 nm,流速0.8 m L/min。并测得青皮中对羟基苯甲醇、3,4-二羟基苯甲酸、对羟基苯甲酸、对羟基苯甲醛的含量分别为（1.3±0.12）、（0.19±0.001）、（0.046±0.003）、（0.15±0.00 9）mg/g。该方法简单、高效、实用。

For the development and utilization of Macadamia green husk, this paper studies the determination method and content of p-hydroxybenzyl alcohol, 3,4-dihydroxy benzoic acid, p-hydroxy benzoic acid and p-hydroxy benzldehyde by HPLC. The results showed that the best determination condition of 4 polyphenols in macadamia husk is 1% acetic acid aqueous solution and methanol as mobile phase in gradient elution（0 min, 87% A; 5 min, 85% A; 25-30 min, 60% A）, detection wavelength is 260 nm and the flow rate is 0.8 m L/min. The content of p-hydroxy benzyl alcohol, 3,4-dihydroxy benzoic acid, p-hydroxy benzoic acid and p-hydroxy benzldehyde in macadamia husk is（1.3±0.12）,（0.19±0.001）,（0.046±0.003）,（0.15±0.009）mg/g. This method is simple, efficient and practical.