茶多酚对铝致大鼠睾丸支持细胞损伤的拮抗作用研究

The antagonistic effects of tea polyphenols on damage of rat sertoli cells induced by Aluminum chloride

目的探讨茶多酚(TP)对铝暴露大鼠睾丸支持细胞损伤的拮抗作用。方法原代培养大鼠睾丸支持细胞。将细胞分为0μmol/L AlCl3(对照组)、400μmol/L AlCl3(铝暴露组)、40μg/mL TP+400μmol/L AlCl3组(低剂量拮抗组)、160μg/mL茶多酚+400μmol/L AlCl3组(高剂量拮抗组),各组用相应的药物处理24h。四甲基偶氮唑盐(MTT)法检测各组细胞的生长活性;逆转录-聚合酶链反应(RT-PCR)检测抑制素B(INHB)mRNA的表达。结果与对照组比较,铝暴露组的细胞活性、INHB mRNA表达均明显下降,差异有统计学意义(P<0.05)。与铝暴露组比较,低剂量拮抗组、高剂量拮抗组能够明显提高细胞活性,差别具有统计学意义(P<0.05)。与铝暴露组比较,高剂量拮抗组能够明显提高INHB mRNA表达,差异有统计学意义(P<0.05)。结论高剂量TP能够拮抗铝对大鼠睾丸支持细胞的损伤。

Objective To observe the antagonistic effects of tea polyphenols on the damage of sertoli cells of rats induced by Aluminum chloride. Methods Rat sertoli cells were cultured,purified and identified. Cells were divided into 0 μmol/L AlCl3 （control group）,400μmol/L AlCl3 （Aluminum exposure group） and 40 μg/mL TP＋400 μmol/L AlCl3 group （low dose anti group） and 160 μg/mL tea polyphenol ＋ 400 μmol/L AlCl3 group （high dose of anti group）, each group with the corresponding drug treatment for 24 h. MTT method was used to detect the growth activity of the cells in each group, the levels of INHB mRNA ex- pression were measured by RT-PCR. Results Compared with the control groups,the cell viability and the level of IN HB mRNA expression of Aluminum exposure groups were obviously decreased, there were statistical significant differences （P〈0.05）. Compared with the Aluminum exposure group, the cell viability of low dose anti group and high dose of anti group was remarkably increased, there were statistical significant differences（P〈0.05）. Compared with the Aluminum exposure group, the level of INHB mRNA express!on of high dose of anti group was remarkably increased, there was statistial significant difference （P〈 0. 05）. Conclusion High dose of tea polyphenols can enhance the expression of mRNA,increase the cell activity, and antagonism Aluminum on the injury of rat＇s testis.