摘要
目的采用Bel-7402人肝癌细胞悬液接种裸鼠构建肝癌裸鼠移植瘤模型,探讨模型体内微量差异蛋白的表达情况。方法在裸鼠皮下接种浓度为1×107/mL的肝癌细胞悬液构建肿瘤模型,剂量为每只0.1mL,对照组接种相同剂量无血清培养液,观察肿瘤生长情况。在不同时间点取血,用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术检测分析裸鼠血清中的小分子蛋白。利用Ciphergen ProteinChip 3.0和Ciphergen Biomaker Wizard 3.1软件进行蛋白质数据采集和分析,筛选与肝癌相关的差异蛋白。结果筛选出115个差异表达蛋白(P<0.01),其中有5个差异蛋白表达规律性较强,质荷比(M/Z)分别为2 016、3 309、3 442、3 745、2 747Da。结论 Bel-7402人肝癌细胞在裸鼠体内生长过程中能够表达与肝癌相关的微量蛋白质,经进一步蛋白鉴定和试剂开发可用作肝癌早期诊断的标志物。
Objective We injected nude mice with human hepatoma cell line （Bel-7402）,in order to analyze their serum microprotein spectrum,and so as to find the early diagnosis markers of liver cancer. Methods We injected every nude mice with cell suspension （concentration： 1 × 10^7/mL;dosage： 0. 1 mL） to built the tumor model, the control group was injected with the same dose of serum free medium. We observed the growth of nude mice and sampled their blood in different time,and then we analyzed the serum microprotein spectrum by SELDI-TOF-MS and screened the proteins related to hepatoma by the embedded software （Ciphergen ProteinChip 3.0 and Ciphergen Biomaker Wizard 3. 1）. Results We got 115 significantly differentiated proteins （P〈 0.01） ,and among them,there were 5 proteins expression regularity, M/Z was 2 016,3 309,3 442,3 745,2 747 Da, respectively. Conclusion Bel-7402 human liver cells in nude mice in vivo growth process has five proteins have difference,further protein identification and reagent developmentcan open up a new way for early diagnosis of liver cancer.
出处
《重庆医学》
CAS
北大核心
2016年第22期3040-3042,共3页
Chongqing medicine
