摘要
目的明确在慢性心房颤动(AF)中Junctophilin-2(JPH2)表达变化及意义。方法从心脏外科体外循环手术中获取21例窦性心律(SR)患者和30例慢性AF患者右心房肌组织,采用免疫组织化学方法测定心房肌组织JPH2蛋白的分布;实时荧光定量PCR法检测心房肌JPH2mRNA水平;Western blot测定人心房肌JPH2蛋白水平。结果免疫组织化学实验发现SR组与AF组心房肌组织上均存在JPH2蛋白表达,SR组着色更明显;AF组心房肌JPH2mRNA水平和蛋白水平均明显低于SR组(P<0.01)。结论慢性AF患者心房肌JPH2的表达下调可能与心房肌细胞内钙紊乱密切相关。
Objective To cxplore the change and significance of Junctophilin-2（JPH2） expression in chronic atrial fibrillation （CAF）. Methods Right atrial muscle tissue of 21 patients with sinus rhythm （SR） and 30 patients with chronic atrial fibrillation （AF） were obtained from cardiopulmonary bypass surgery. The distribution of JPH2 in human atrial was assayed by immunohistochemistry technique; mRNA expression level was measured by the qRT-PCR, and the protein expression level of JPH2 was determined using Western blot analysis. Results Immunohistochemistry experiments found that the expression of JPH2 protein was found in right atrial muscle tissue of SR group and AF group, SR group staining was more obvious ;JPH2 mRNA levels and protein levels were significantly lower in group AF than in SR group（P〈0.01）. Conclusion JPH2 is widely distributed in atrial muscle cells of SR group and AF group,the expression of JPH2 in patients with CAF may be closely related to the atrial myocytes calcium disorder.
出处
《重庆医学》
CAS
北大核心
2016年第22期3046-3048,共3页
Chongqing medicine
基金
国家自然科学基金资助项目(81470022)
泸州市-泸州医学院联合项目(2013LZLY-J23)
