口腔白斑和鳞状细胞癌中人乳头瘤病毒感染分析

Human papillomavirus infection in oral leukoplakia and oral squamous cell carcinoma

目的探讨人乳头瘤病毒(HPV)16/18感染与口腔鳞状细胞癌(OSCC)临床病理特征间的关系。方法原位杂交(ISH)和免疫组织化学(IHC)分别检测10例正常口腔黏膜,26例口腔白斑(OLK)和56例OSCC中的HPV16/18水平,分析不同组间HPV16/18阳性率间的差异和OSCC中HPV16/18感染与性别、年龄、TNM分期、组织学分化、淋巴结转移、吸烟的关系。结果 ISH和IHC检测HPV16/18阳性率差异无统计学意义(P>0.05);OSCC和OLK患者中HPV16/18阳性率高于正常口腔黏膜(P<0.05);OSCC中吸烟者HPV16/18阳性率明显高于非吸烟者(P<0.05)。结论 HPV16/18感染是部分OSCC的发病因素,其与吸烟对OSCC发病具有协同作用。

Objective To investigate the correlation between HPV16/18 infection and the clinicopathological characteristics of oral squamous cell carcinoma （OSCC）. Methods HPV16/18 was detected by the situ hybridization （ISH） and immunohistochemical （IHC） in 10 cases of normal oral mucosa,26 cases of oral leukoplakia （OLK） and 56 cases of oral squamous cell carcinoma （OSCC）. We analysed the difference of the HPV16/18 positive rate among groups,and explored the correlation between HPV infection and the gender,age,TNM staging,histological garde,lymph node metastasis and smoking in OSCC patients. Results There was no significant difference between ISH and IHC in detecting HPV16/18 positive rate （P〉0.05）. The HPV16/18 positive rates in patients of OLK and OSCC were significantly higher than that in normal oral mucosa patients（P〈0.05）. The HPV16/18 positive rate in smokers was significantly higher than that in non-smokers of OSCC（P〈0.05）. Conclusion HPV16/18 infection is considered to be a pathogenic factor in OSCC. HPV16/18 infection and smoking have a synergistic effect on the pathogenesis of OSCC.