水通道蛋白-7在小鼠肾集合管发育过程中的表达分析

Analysis on expression of Aquaporin-7 in mouse renal collecting duct during development

目的探讨水通道蛋白-7(aquaporin-7,AQP-7)在小鼠肾集合管发生发育过程中的表达和作用。方法采用免疫组化结合体视学测量和免疫印迹技术,测定胚龄(E)16、18d胎鼠及生后(P)1、3、5、7、14、21、42、70d仔鼠肾集合管中AQP-7的表达及含量变化。结果通过体视学测量和免疫印迹技术结果显示:AQP-7在胚龄16d的小鼠肾集合管开始弱表达,到生后21d达到高峰,之后随着日龄的增加,表达无明显变化。结论 AQP-7主要表达在发育中小鼠肾集合管中,可能和肾集合管的发育和成熟相关。

Objective To explore the expression and function of aquaporin-7（AQP-7） in mouse renal collecting duct during kidney development. Methods Immunohistochemical,stereological measurements and western blot were used to detected the expression and level of AQP-7 in the renal collecting duct of mice on embryonic day（E）16,18 d and postnatal kidney（P） 1,3,5,7,14,21,42,70 d. Results By stereological and Western blot,it was showed that AQP-7 weakly expressed in collecting duct of embryonic 16 day mouse kidney,at 21 days after birth,it reach the peak.Then with age increasing,its expression had no significant change. Conclusion AQP-7 was expressed mainly in the developing mouse kidney collecting duct,and it may be related to renal collecting duct development and maturation.