木瓜蛋白酶处理鱼粉代乳对6～19日龄羔羊采食与消化代谢的影响

Effects of Papain-treated Milk Substitute on the Intake,Digestion and Metabolism of 6-19 Days Age Lambs

将18只初生体重为（3.25±0.61）kg的小尾寒羊母羔随机分为3组,每组6只,从3日龄起分别补饲牛奶粉代乳、鱼粉（50%）代乳和木瓜蛋白酶预处理鱼粉（50%）代乳,从5日龄完全脱离母乳依靠代乳,在6～9日龄及16～19日龄进行二次消化代谢实验。结果表明,在6～9日龄,牛奶粉代乳组、50%鱼粉代乳组、木瓜蛋白酶预处理鱼粉（50%）代乳组羔羊的干物质自由采食量分别为（147.6±31.2）、（74.0±17.5）和（98.7±19.6）g/d;干物质表观消化率分别为（96.8±1.0）%、（80.8±7.6）%和（88.0±3.6）%;粗蛋白表观消化率分别为（93.1±3.7）%、（71.1±8.2）%和（77.3±9.7）%;氮保留分别为（2.75±0.90）、（0.79±0.63）和（1.44±0.65）g/d;日增重分别为（143.3±44.4）、（26.0±12.3）和（33.1±7.9）g/d。在16～19日龄,牛奶粉代乳组、50%鱼粉代乳组、木瓜蛋白酶预处理鱼粉（50%）代乳组羔羊的干物质采食量分别为（223.5±81.2）、（79.8±18.8）和（107.2±19.6）g/d;干物质表观消化率分别为（97.2±0.6）%、（74.0±4.4）%和（87.8±3.1）%;粗蛋白表观消化率分别为（93.1±3.4）%、（66.4±7.6）%和（77.5±6.1）%;氮保留分别为（4.29±1.97）、（0.81±0.44）和（1.50±0.49）g/d;日增重分别为（198.1±91.7）、（26.8±13.6）和（51.0±15.1）g/d。本研究表明,饲喂经木瓜蛋白酶处理的鱼粉代乳可提高羔羊的自由采食量、干物质和粗蛋白的消化率、氮保留和日增重,但仍显著低于饲喂牛奶粉代乳的羔羊,酶处理鱼粉代乳的实际应用仍不成熟。

Eighteen Small Tail Han sheep lambs,with birth body weight（3.25±0.61）kg,were randomly divided into 3groups（n =6in each group）,and from 3days age,fed with the milk substitutes of dried milk group,fish meal（50%）group or papain-treated fish meal（50%）group respectively.The lambs lived on the substitute completely from 5days age,and two experiments of the digestion and metabolism were carried on at 6-9and 16-19 days age.The results showed that 6-9days age,in dried milk group,fish meal（50%）group or papain-treated fish meal（50%）group the voluntary intake of dry matter（DM）by the lambs were（147.6±31.2）,（74.0±17.5）and（98.7±19.6）g/d respectively;the apparent digestibility（AD）of DM were（96.8±1.0）%,（80.8±7.6）% and（88.0±3.6）% respectively;the AD of crude protein were（93.1±3.7）%,（71.1±8.2）% and（77.3±9.7）%respectively;nitrogen retention（NR）were（2.75±0.90）,（0.79±0.63）and（1.44±0.65）g/d respectively;and the daily body weight gain（DBWG）were（143.3±44.4）,（26.0±12.3）and（33.1±7.9）g/d respectively.At the 16-19 days age,in dried milk group,fish meal（50%）group or papain-treated fish meal（50%）group the voluntary intake of DM by the lambs were（223.5±81.2）,（79.8±18.8）and（107.2±19.6）g/d respectively;the AD of DM were（97.2±0.6）%,（74.0±4.4）% and（87.8±3.1）%respectively;the AD of crude protein were（93.1±3.4）%,（66.4±7.6）%and（77.5±6.1）%respectively;NR were（4.29±1.97）,（0.81±0.44）and（1.50±0.49）g/d respectively;and the DBWG were（198.1±91.7）,（26.8±13.6）and（51.0±15.1）g/d respectively.It is concluded that feeding the substitute of papain-treated fish meal to the lambs could increase the voluntary intake,the AD of the DM and crude protein,NR and DBWG,however the papain-treated fish meal substitute was still poorer than that the dried milk substitute,and therefore the application of the protease-treated fish meal substitute is not yet available.