百草枯对人肝HepG2细胞存活力和凋亡的影响

Effects of Paraquat on Viability and Apoptosis in Human Hepatic HepG2 Cells

探讨百草枯(PQ)对人肝细胞的毒性。采用甲基噻唑蓝试验和流式细胞术分别检测了PQ处理24 h对人肝Hep G2细胞存活力和凋亡的影响。PQ处理24 h时,对Hep G2细胞存活力的半数抑制浓度为51.17 mg/L;7.21 mg/L的PQ处理24 h即可启动Hep G2细胞的早期凋亡程序,随着PQ处理浓度的增大,Hep G2细胞的总凋亡率先升高后降低,而细胞坏死率持续升高。PQ对人肝Hep G2细胞具有明显毒性,会抑制Hep G2细胞的存活力,低浓度时主要诱导细胞凋亡,高浓度则主要导致细胞坏死,说明保肝类药物可能对PQ中毒有较好疗效。

The cytotoxicity of paraquat（PQ） on human liver was explored comprehensively. Changes of viability and apoptosis were determined respectively by methyltetrazolium assay and flow cytometry in human hepatic Hep G2 cells after 24 h PQ treatment. 24 h median inhibitory concentration of viability was 51.17 mg / L in Hep G2 cells treated by PQ. Early apoptotic Hep G2 cells were observed in 7.21 mg / L PQ treatment group. The total apoptotic rate of Hep G2 cells went up at first and went down at last, but the necrotic rate kept a continuous increasing tendency with the increase of PQ treatment concentrations. PQ had a heavy toxicity on human hepatic Hep G2 cells, inhibited cell viability, induced mainly cell apoptosis at a low concentration and cell necrosis at a high concentration, and which suggested liver protection drugs were possible to be effective for the treatment after PQ poisoning.