摘要
目的建立炎热清片中龙胆苦苷含量的反相高效液相色谱法。方法色谱柱采用Agilent He—C18柱(250mm×4.6mm,5μm);流动相甲醇-0.4%磷酸(10:90—60:40,0—30mim),梯度洗脱;流速1.0mL/min;检测波长270nm;柱温25℃。结果龙胆苦苷进样量在0.2208—1.6470μg范围内与峰面积呈良好的线性关系(r=0.9997);平均回收率为97.19%,RSD为1.07%(n=6)。结论该方法简便,可行、重现性好,可用于炎热清片中龙胆苦苷的含量测定。
Objective To establish an RP- HPLC method for the content determinate of gentiopicroside in Yanreqing Tablets. Methods The Agilent Hc-C18(250 mm×4. 6 mm, 5 μm) chromatography column was used, gradient elution was carried out with methanol-0. 4% phosphoric acid(10 : 90→60:40,0→30 mim) as mobile phase, the flow rate was 1.0 mL/min, the detection wavelength was 270 nm, the column temperature was 25 ℃. Results The calibration curve of gentiopicroside was in a good linearity in the range of 0. 220 8- 1. 647 0 μg( r = 0. 999 7). The average recovery rate was 97.19%, RSD was 1.07% (n = 6). Conclusion The method is simple, feasible and reproducible, and it can be used in the content determination of gentiopicroside in Yanreqing Tablets.
出处
《中国药业》
CAS
2016年第15期71-73,共3页
China Pharmaceuticals