中国汉族人群COPD吸烟史患者HHIP基因多态性与肺癌的相关性研究

Association of HHIP gene polymorphism and lung cancer in Chinese Han population with COPD smoking history

目的探讨中国汉族人群COPD吸烟史患者HHIP基因多态性与发生肺癌风险的相关性。方法以672例COPD患者作为研究对象,依据病情分为试验组(COPD合并肺癌患者,n=265)和观察组(单纯COPD患者,n=207),采用连接酶检测反应法对患者HHIP基因的多态性位点rs1489758、rs1489759、rs10519717、rs13131837、rs1492820、rs7689420进行多态性分析。对连锁不平衡性分析及SNP点基因型与肺癌风险进行Logistic回归分析。结果HHIP基因位点rs1489758、rs1489759、rs10519717、rs13131837、rs1492820、rs7689420经Hardy·Weinberg平衡检验,均符合H.W平衡定律(P>0.05),具有群体代表性。以是否合并肺癌为因变量,各研究位点基因型多态性为自变量,行Logistic回归分析(年龄、性别、吸烟状况进行校正),结果显示,HHIP基因位点rs1489758、rs1489759、rs10519717、rs13131837、rs1492820、rs7689420与COPD患者是否并发肺癌无统计学关系(P>0.05)。结论本研究未发现HHIP基因的6个多态性位点与中国汉族COPD患者的肺癌发病风险有明确相关性。

Objective To investigate the association of HHIP gene （protein Hedgehog-interacting, HHIP） polymorphism with the risk of lung cancer in Chinese Han population with COPD smoking history. Methods 672 patients with COPD were used as the research object. According to the condition of the patients, the patients were divided into test group （patients with COPD and lung cancer, n= 265） and observation group （patients with unilateral chronic obstructive pulmonary disease （COPD）, n=207）. By using the ligase detection reaction （ligase detection reaction, LDR）, polymorphism of HHIP gene at rs1489758, rs1489759, rs10519717, rs13131837, rs1492820 and rs7689420 were detected. SNP genotype and lung cancer risk were analyzed. Results HHIP of rs1489759, rs10519717, rs13131837, rs7689420, rs1492820 and rs1489758 were accord with Hardy · Weinberg equilibrium test （P〉0.05）, which were representative of the population. Logistic regression analysis showed that HHIP gene polymorphism of rs1489758, rs1489759, rs10519717, rs13131837, rs1492820 and rs7689420 were not related to and COPD patients with lung cancer （P〉0.05）.Conclusion There is a clear correlation between the 6 polymorphisms of HHIP gene and the risk of lung cancer in Chinese Han COPD patients.