星形细胞上调基因-1在原发性肝细胞癌组织中的表达及意义

Expression and significance of astrocyte elevated gene-1 gene in primary hepatocellular carcinoma

目的探讨星形细胞上调基因-1(astrocyte elevated gene-1,AEG-1)在原发性肝细胞癌组织中的表达及其意义。方法 60例肝细胞癌患者,取其肝癌组织60份为肝癌组,癌旁肝组织54份为对照组,采用荧光定量PCR法检测2组AEG-1mRNA表达情况,采用Western blot法检测AEG-1蛋白表达情况,采用免疫组织化学法检测AEG-1阳性表达率,分析AEG-1蛋白表达与病理指标间的关系。结果肝癌组AEG-1mRNA和蛋白相对表达量(4.71±2.87、1.27±0.62)及AEG-1阳性表达率(53.3%)均明显高于对照组(1.43±0.49、0.69±0.25、29.6%)(P<0.05);肝癌组织AEG-1阳性表达水平在肿瘤转移及病理分级上差异无统计学意义(P>0.05)。结论 AEG-1在原发性肝细胞癌组织中特异性表达增高,可能是诊断原发性肝细胞癌的生物学标记物。

Objective To investigate the expression of astrocyte elevated gene-1 （AEG-1） in primary hepatocellular carcinoma （HCC） and its clinical significance. Methods The expression of AEC-1 mRNA was detected by fluorescence quantitative PCR technique, AEG-1 protein was detected by Western blot method, and the positive rate of AEG-I was detected by immunohistochemical method in 60 samples of HCC tissue （HCC group） and 54 samples of adjacent tissue （control group） from 60 patients with HCC. The relationship between the expression of AEG-1 gene and pathological index was analyzed. Results The relative expressions of AEG-1 mRNA and protein and the positive expression rate of AEG-1 protein were significantly higher in HCC group （4.71±2.87, 1.27±0.62, 53.3%） than those in control group （1.43±0.49, 0.69±0.25, 29.6%） （P〈0.05）. There was no significant difference in the positive expression level of AEG -1 in tumor metastases and pathological grading in HCC group （P〉0.05）. Conclusion AEG 1 is highly expressed in primary HCC tissue, suggesting that AEG-1 may be regarded as a biomarker for the diagnosis of primary HCC.