基质细胞衍生因子对人视网膜微血管内皮细胞的作用

Effects of SDF-1 on proliferation and migration of human retinal capillary endothelial cells

目的观察基质细胞衍生因子(stromal cell-derived factor-1,SDF-1)对体外培养的人视网膜微血管内皮细胞(human retinal capillary endothelial cells,HRCECs)增殖和迁移活性的影响。方法体外培养HRCECs,以MTT法和Transwell实验分别检测SDF-1和VEGF干预下细胞增殖、迁移活性的改变,二者的浓度分别为5 ng·m L^(- 1)、50 ng·m L^(- 1)、100 ng·m L^(- 1)。联合5 ng·m L^(- 1)VEGF和100 ng·m L^(- 1)SDF-1观察二者的相互作用。结果VEGF促细胞增殖呈浓度依赖型,5 ng·m L^(- 1)、50 ng·m L^(- 1)和100 ng·m L^(- 1)组吸光度值分别为0.304±0.033、0.417±0.001和0.447±0.025,均明显大于阴性对照组0.207±0.003(均为P<0.05);SDF-1作用组吸光度值分别为0.21±0.03、0.22±0.05、0.21±0.11,与阴性对照组相比均无明显改变(均为P>0.05)。100 ng·m L^(- 1)SDF-1联合5 ng·m L^(- 1)VEGF共同作用时,细胞增殖活性(0.47±0.07)与阴性对照组相比明显增高(P<0.01)。HRCECs迁移活性呈SDF-1浓度依赖性,在50 ng·m L^(- 1)和100 ng·m L^(- 1)时,细胞迁移计数分别为480.0±35.6和700.0±71.2,与阴性对照组相比均有显著性差异(均为P<0.01),联合5 ng·m L^(- 1)VEGF促迁移活性明显增加。结论 SDF-1可以促HRCECs迁移,并提高VEGF促HRCECs的增殖作用。

Objective To analyze the effects of stromal cell-derived factor-1（ SDF-1） on proliferation and migration of human retinal capillary endothelial cells（ HR C EC s） in vitro. Methods HR C EC s were cultured in vitro. C ell proliferation was studied by MTTunder different concentrations（ 5 ng·m L（- 1）,50 ng·m L（- 1）and 100 ng·m L（- 1）） of SDF-1 and VEGF,and Transw ell was used to evaluate chemotaxis effect of SD F-1 on HR C EC s. The interactive effect of100 ng·m L（- 1）SD F-1 and 5 ng · m L（- 1）VEGF was observed. Results O n HR C EC s,VEGF induced a dose-dependent increase in cell proliferation,O D value of 5 ng·m L（- 1）,50 ng·m L（- 1）and 100 ng·m L（- 1）VEGF groups were 0. 304 ± 0. 033,0. 417 ± 0. 00 1and 0. 447 ± 0. 025,respectively,which were higher than the control group（ 0. 207 ± 0. 003）（ all P〈 0. 05）. O D value of 5 ng·m L（- 1）,50 ng·m L（- 1）and 100 ng·m L（- 1）SD F-1 groups were 0. 21 ± 0. 03,0. 22 ± 0.05,0. 21 ± 0. 11,respectively,there was no statistical difference compared with the control group（ all P〉 0. 05）. However,100 ng·m L（- 1）SD F-1 combined with 5 ng·m L（- 1）VEGF promoted a significant increase in proliferation（ 0. 47 ± 0. 07） compared with the control group（ P〈 0. 01）.SD F-1 led a dose-dependent increase in cell migration,which significantly increased in 50 ng ·m L（- 1）and 100 ng·m L（- 1）groups with cell count of 480. 0 ± 35. 6 and 700. 0 ± 71. 2,respectively,there was significant difference compared with the control group（ P 〈 0. 01）; And this effect could be amplified when combination with 5 ng·m L（- 1）VEGF. Conclusion SD F-1 can promote the migration of HR C EC s,and enhance the proliferative effect of VEGF.