铁苋菜中原儿茶酸和原儿茶醛含量的高效液相色谱法测定

Determination of Protocatechuic acid and Protocatechuic aldehyde in Acalypha australis L. by HPLC

目的建立RP-HPLC测定不同产地铁苋菜中原儿茶酸和原儿茶醛的含量。方法依利特ODS C_（18）（4.6 mm×250mm,5μm）,以甲醇-0.1%磷酸水溶液（25︰75）为流动相,流速：1.0 ml/min;检测波长：260 nm;柱温：25℃。结果原儿茶酸在0.09~1.8μg范围内线性关系良好（r=0.9996）,平均回收率为99.62%（RSD=0.41%）;原儿茶醛在0.03~0.6μg范围内线性关系良好（r=0.9999）,平均回收率为99.45%（RSD=1.18%）。结论该方法简便、快速、准确可靠,可为铁苋菜的质量控制提供依据。

Objective To establish an RP- HPLC determination of Protocatechuic acid and Protocatechuic aldehyde in Acalypha australis L. from different sources. Methods The separation was performed on a Hypersil ODS C_（18）（ 4. 6 mm × 250 mm,5 μm）column,methanol- 0. 1% phosphoric acid（ 25∶ 75） as the mobile phase; The flow rate was 1. 0 mL / min; The detection wavelength was 260 nm and the column temperature was at 25 ℃. Results Linear ranges of Protocatechuic acid and Protocatechuic aldehyde were 0. 09 ～ 1. 8 μg（ r = 0. 9996）,0. 03 ～ 0. 6 μg（ r = 0. 9999）. The average recoveries were 99. 62 %（ RSD = 0. 41 %）and 99. 45 %（ RSD = 1. 18 %）（ n = 6）,respectively. Conclusion The method is simple,rapid,accurate and which can be applied as a standard for the quality control of Acalypha australis L.