解整合素-金属蛋白酶10基因与儿童哮喘的相关性分析

Association between ADAM10 polymorphism and clinical characteristics in children with asthma

目的 探讨我国中部地区儿童中ADAM 10基因的2个SNP位点,rs 653765和rs 514049的多态性与哮喘及其临床特点的相关性。方法 采用病例对照研究方法,选取221例哮喘患儿和236例同期体检正常儿童作为研究对象,利用限制性片段长度多态性分析（PCR-RFLP）方法检测2个SNP位点的多态性分布;采用ELISA方法测定血浆中的ADAM 10的水平。结果 哮喘患儿rs 653765的三种基因型（CC、TC、TT）与正常对照组差异具有统计学意义（χ~2=7.163,P=0.028）;等位基因频率的差异也具有统计学意义（P=0.008）,T等位基因为风险因子。哮喘患儿rs514049的基因型以及等位基因频率与对照组差异无统计学意义（χ~2=1.011~0.844,P均〉0.05）。哮喘组和对照组之间血清中ADAM10水平的差异无统计学意义（t=1.233,P=0.238）;哮喘患儿中,rs653765为TT基因型患儿的ADAM10水平高于CC基因型患儿,差异有统计学意义（P=0.034）。结论 ADAM10基因SNP位点（rs653765）的多态性与我国中部地区儿童哮喘的易感性相关;且该位点多态性可能影响患儿ADAM10水平。

Objective To investigate the association between single nucleotide polymorphisms （SNP） rs 653765 and rs 514049 in ADAM10 gene and clinical characteristics in asthma in Han Chinese children. Methods A case-control study was performed in that research, and 221 children with asthma were recruited and an other 236 normal children were recruited as controls. The genotypes of two SNPs in ADAM10 gene were detected using PCR-RFLP. And all data were analyzed by SPSS19 . 0 . Results In case-controlled study, we found the frequency of TT genotype in case group was higher than that of controlgroup （P = 0 . 028 ）, and the frequency of T allele was higher than that of control group （P = 0 . 008 ）. The genotypes and alleles of rs 514049 was not associated with asthma （P = 0 . 604 , 0 . 356 ）.There were no difference in serum ADAM 10 levels between asthma and control group （P = 0 . 238 ）, but in asthma group we found patients with TT genotype may have a higher level ADAM 10 than the one with CC genotype （P = 0 . 034 ）. The polymorphism of rs 653765 was not associated with the level of C-reaction protein, the number of LYM, IgE and EOS% （P 〉 0 . 05 ）. Conclusions The genotype of rs 653765 in ADAM10 gene was associated with asthma in the central of China, and T allele was a risk factor. The polymorphism of rs 653765 might be associated with the levels of ADAM 10 .