益艾康胶囊对大鼠体内奈韦拉平药动学的影响

Effect of Yiaikang capsules on pharmacokinetics of nevirapine in rats

目的：研究益艾康胶囊对奈韦拉平在大鼠体内药动学的影响,为益艾康胶囊在临床合理用药提供依据。方法：实验大鼠分别灌胃给予奈韦拉平和奈韦拉平＋益艾康胶囊,并通过LC/MS/MS测定2种给药方式后血浆中奈韦拉平浓度,随后使用Phoenix Win Nonlin 6.1软件计算药动学参数。结果：符合方法学考察要求：血药浓度在5～500 ng·mL^-1范围内线性关系良好,日内和日间精密度均〈10.0%,提取回收率89.8%～107.0%,稳定性较好,受基质影响较小。由2组药动学主要参数得知MRT0～t和MRT0～∞均显著升高（P〈0.05）,Cmax显著降低（P〈0.05）,其他参数无统计学意义。结论：益艾康胶囊确实对奈韦拉平在大鼠体内药动学产生了影响。

Objective： To study the effect of Yiaikang（ YAK） capsules on the pharmacokinetics of nevirapine（ NVP） in rats and provide the basis for its rational use in clinic. Methods： HPLC-MS / MS was used to measure the NVP concentration in the plasma of rats after intragastric administration of NVP and NVP ＋ YAK,respectively. Then the pharmacokinetic parameters were calculated by Phoenix Win Nonlin 6. 1. Results： The HPLC-MS / MS method met the requirements of the methodological well. The calibration curve for plasma NVP had good linearity in the concentration range of 5 ～ 500 ng·mL^-1. The intar-day and inter-day RSDs were less than10. 0%,abd the extraction and recovery rates ranged from 89. 8% to 107. 0%. The data indicated that the stability of the samples was good and there was little substrate effect. The main pharmacokinetic parameters of NVP,MRT 0 ～ t and MRT 0 ～ ∞,were significantly increased in the NVP ＋ YAK group（ P〈0. 05）,and Cmaxwas significantly decreased（ P〈0. 05）. However,the difference in the other pharmacokinetic parameters showed no statisticalsignificance. Conclusion： Yiaikang capsules have significant effect on the pharmacokinetics of nevirapine in rats.