动态浸提提取栀子黄色素工艺研究

Study on Extraction Technology of Gardenia Yellow Pigment by Dynamic Extraction

以除油栀子粉为原料,乙醇水溶液为浸提溶剂,进行乙醇回流动态浸提提取栀子黄色素,考察各个因素对栀子黄色素的提取率的影响,并与传统浸提提取工艺对比,说明动态浸提提取工艺的先进性。通过单因素试验和正交试验确定最佳工艺条件为:60%乙醇溶液为浸提溶剂、溶液pH值7.0、提取温度60℃、每次提取时间1h、固液比1∶10g/mL、提取次数2次,在此条件下栀子黄色素的提取率达到94.23%,精制后栀子黄色价70、栀子苷含量0.2%(以色价10计进行换算);同时得到栀子苷和绿原酸,提取率分别为90.81%和85.32%。

Taking Gardenia powder removing oil as raw material and ethanol solution as solvent, the extraction of gardenia yellow pigment by ethanol reflux extraction was carried out, to study the various factors influence on the rate of extraction of gardenia yellow pigment, And compared with the traditional extraction process , the advanced nature of the extraction process of dynamic extraction is described. Through single factor test and orthogonal test, the optimum conditions were determined as follows： 60% ethanol solution as extraction solution, solution pH value 7.0, extraction temperature 60 degrees C, extraction time 1 hours, solid-liquid ratio 1 ：10g/ml, extraction times 2 times, Under these conditions, the extraction of gardenia yellow pigment rate reached 94.23%, 70 gradation, gardenia glycoside content of 0.2% （to gradation 10 conversion）, and obtained geniposide and chlorogenic acid, extraction rate was 90.81% and 85.32 % respectively.