摘要
目的建立名草口服液A型、B型中二苯乙烯苷的含量测定方法。方法采用高效液相色谱法(HPLC),Waters XBridge C18柱(4.6mm×250mm,5pm);以乙腈.水(16:84)为流动相;检测波长为320nm。结果二苯乙烯苷在19.64-147.31ng范围内线性关系良好,相关系数r=0.9995,A型平均回收率98.9%,B型平均回收率99.2%。结论该方法简单易操作,准确可靠,可用于名草口服液A型、B型的质量控制。
Objective To establish the method for determination of the content of stilbene glucoside in Mingcao Oral Liquid Type A and Type B. Methods The content of stilbene glucoside was determined by HPLC. Waters XBridge C18 was adopted, using aeetonitrile-water (16:84) as the mobile phase, and the detection wavelength was 320 nm. Results Stilbene glucoside had a good linear relationship in the range of 19.64-147.31 ng, with the correlation coefficient (r) of 0.9995. The average recoveries of stilbene glucoside in Mingcao Oral Liquid Type A and Type B were 98.9 % and 99.2 %, respectively. Conclusion The method is simple and easy to operate, and accurate and reliable, thus can be used for the quality control of Mingcao Oral Liquid Type A and Type B.
出处
《食品与药品》
CAS
2016年第4期276-279,共4页
Food and Drug
基金
贵州省科技基础条件平台课题(编号:黔科平台[2012]4001)