草莓茎尖组织培养和快繁体系的建立

Tissue Culture and Rapid Propagation of Strawberry

以草莓主栽品种鬼怒甘的茎尖为材料,研究了Hg Cl2浓度及其消毒时间、基础培养基、白砂糖、6-苄氨基腺嘌呤（6-BA）、萘乙酸（NAA）、吲哚丁酸（IBA）、吲哚乙酸（IAA）、活性炭（AC）等9种主要因素对草莓茎尖组织培养的影响,分析筛选出了一套能够产出具有优良素质草莓种苗的组织培养技术,建立起了比较完善的草莓茎尖组织培养快繁体系。结果表明：不同因素对草莓茎尖组织培养的影响较大,尤其是影响草莓的形态发育（分化、增殖、生根和生长）。最佳外植体消毒方法是0.20%Hg Cl2 8-11min,适合初代培养的培养基为MS＋6-BA 0.5-1.0mg/L＋NAA 0.01mg/L＋白砂糖30g/L,增殖继代培养基为MS＋6-BA 0.5-1.0mg/L＋NAA 0.01-0.05mg/L＋白砂糖20-30g/L,生根培养基为MS＋NAA 0-0.01mg/L＋IBA 0-0.1mg/L＋IAA 0-0.1mg/L＋白砂糖30g/L。

Taking one main strawberry variety of Guinugan as sample,experiments were carried out to study the effects of 9 factors（Hg Cl2,basal medium,white granulated sugar,etc.） on tip-shoot tissue culture of strawberry,and then to screen out a tip-shoot tissue culture technology for strawberry,which could produce better quality seedlings,and eventually to establish a relatively complete tip-shoot tissue culture and rapid propagation system in strawberry. The results showed that 9 factors had great effects on strawberry tip-shoot tissue culture.The best sterilization approach for explants was to treat 8-11 min by 0.20% Hg Cl2,the suitable primary culture medium was MS＋6-BA 0.5-1.0mg/L＋NAA 0.01mg/L＋white granulated sugar 30g/L,the suitable proliferation medium was MS＋6-BA 0.5-1.0mg/L＋NAA 0.01-0.05mg/L＋white granulated sugar 20-30g/L,and the suitable rooting medium was MS＋NAA 0-0.01mg/L＋IBA 0-0.1mg/L＋IAA 0-0.1mg/L＋ white granulated sugar 30g/L.