两种陈皮中橙皮苷含量测定供试品溶液制备方法的比较

the method comparison of two kinds of solution preparation method for hesperidin content measure in pericarpium citri reticulatae

目的:改进和完善陈皮中橙皮苷含量测定供试品溶液制备方法。方法:用石油醚回流后弃去石油醚液,用移液管精密加入甲醇,称定重量后回流提取1.5h,用甲醇补足减失重量,摇匀,滤过取续滤液作为供试品溶液,与药典制备法进行方法学比较。结果:改进后的制备方法测定陈皮中橙皮苷含量优于药典制备方法。结论:改进后的方法操作更简便、准确度更高、重复性更好,可用于陈皮中橙皮苷含量的检测。

Objective： To improve and perfect the method of the test solution preparation for hesperidin determination in pericarpium citri reticulatae . Methods： Petroleum ether relfux discarded petroleum ether solution, with a pipette to add methanol precision, relfux extraction for 1.5 hours after weighing amount , and add the loss of weight by methanol , shaking up, after ifltering take the continued ifltrate as the test solution, methodological comparison Pharmaceutical Preparation. Results： The preparation of the improved measurement Citrus hesperidin content is better than Pharmaceutical preparation.Conclusion： The improved method is more simple、more accurate and more reproducible and can be used to detect hesperidin in pericarpium citri reticulatae.