大肠杆菌表达系统和酵母表达系统的研究进展

Research Progress of Expression Systems of Escherichia coli and Yeast

由于DNA重组技术和蛋白组技术的发展与成熟,外源基因表达技术备受关注。其在生物、食品、工业以及医学领域发挥着举足轻重的作用。以原核生物细胞及真核生物细胞作为表达系统,进行目的基因序列的表达以生产蛋白疫苗、核酸疫苗和生物酶制剂等是近年来发酵工业的新型领域。原核和真核表达系统是近年来研究和应用最广泛和最成熟的外源基因表达系统。对近年来关于大肠杆菌表达系统和酵母表达系统的研究进展进行了综述。

Due to the rapid development of recombinant DNA technology and proteomic technology , exogenous gene expression technology has received much attention.It plays increasingly important role in the field of biological, foodstuff, industry and medical science.In recent years , the use of prokaryotic and eukaryotic expression systems for exogenous gene amplification and expression to produce proteins vaccines , nucleic acid vaccine and enzyme preparations, etc.is the emerging fields of fermentation industry.Prokaryotic expression and eukaryotic ex-pression systems were commonly used to express exogenous gene.The research progress of yeast and E.coli expression systems in recent years were reviewed.