假蒟不同部位5种次生代谢产物含量测定

Determination of Five Kinds of Secondary Metabolites in Different Parts of Piper sarmentosum Roxb.

[目的]建立假蒟不同部位总黄酮、总生物碱、总皂苷、总酚和总鞣质含量的测定方法。[方法]通过Na NO2-Al(NO3)3-Na OH法测定总黄酮含量;通过溴甲酚绿酸性染料比色法测定总生物碱含量;通过香草醛-冰醋酸酸性染料比色法测定总皂苷含量;通过FolinCiocalteu比色法测定总酚含量;通过磷钼钨酸-干酪素比色法测定总鞣质含量。[结果]芦丁平均加样回收率为98.11%,RSD=0.59%,假蒟叶、茎、根中总黄酮含量分别为1.18%、0.33%、0.25%;盐酸小檗碱平均加样回收率为99.62%,RSD=1.52%,假蒟叶、茎、根中总生物碱含量分别为0.19%、0.03%、0.01%;人参皂苷Re平均加样回收率为99.21%,RSD=1.06%,假蒟叶、茎、根中总皂苷含量分别为4.33%、1.71%、4.13%;没食子酸平均加样回收率为97.95%,RSD=0.88%,假蒟叶、茎、根中总酚含量分别为2.42%、1.08%、0.68%;没食子酸平均加样回收率为97.92%,RSD=3.14%,假蒟叶、茎、根中总鞣质含量分别为1.69%、1.21%、0.72%。[结论]这5种方法准确性强、精密度高、重复性好,可以用作假蒟中总黄酮、总生物碱、总皂苷、总酚和总鞣质含量的测定。

Objective] To establish the analytical methods for detecting the contents of total flavonoids, total alkaloids , total saponins, total phenolics and total tannins in different parts of Piper sarmentosum Roxb.[ Method] The content of total flavonoids was measured by the NaNO2A-l（ NO 3） 3-NaOH colorimetry.The content of total alkaloids was measured by the bromocresol green acid dyes colorimetric assay .The content of total spaonins was measured by the vanillin-acetic acid dyes colorimetric assay.The content of total phenolics was measured by the Folin-Ciocalteu assay.The content of total tannins was measured by the phospho molybdenum tungstic acid -casein colorimetry.[ Result] The average recovery of rutin was 98.11% and RSD was 0.59%, the contents of total flavonoids in roots, stems and leaves of P.sarmentosum were 0.25%, 0.33% and 1.18% respectively.The average recovery of berberine hydrochloride was 99.62% and RSD was1.52%, the con-tents of total alkaloids in roots, stems and leaves of P.sarmentosum were 0.01%, 0.03% and 0.19% respectively.The average recovery of ginsenoside Re was 99.21% and RSD was 1.06 %, the contents of total saponins in roots, stems and leaves of P.sarmentosum were 4.13%, 1.71% and 4.33% respectively.The average recovery of gallic acid was 97.95% and RSD was 0.88 %, the contents of total phenol in roots, stems and leaves of P.sarment soum were 0.68%, 1.08% and 2.42% respectively.The average recovery of gallic acid was 97.92%and RSD was 3.14%, the contents of total tannins in leaves, stems and roots of P.sarmentosum were 0.72%, 1.21% and 1.69% respec-tively.[Conclusion] These five proposed methods are precise, accurate and reproducible, and they are suitable for the determination of total flavonoids, total alkaloids, total saponins, total phenolics and total tannins in P.sarmentosum.