摘要
目的通过测定肺癌患者化疗前后不同时间点外周血淋巴细胞的变化,探讨化疗后各类型淋巴细胞的恢复特点以及临床和病理方面的影响因素。方法采用流式细胞术检测非小细胞肺癌患者(87例)在化疗前1周、化疗后2周、3个月和9个月外周血NK、CD8^+、CD4^+T淋巴细胞和B淋巴细胞的水平,分析化疗前后淋巴细胞亚群的变化,并根据化疗方案及临床特点的不同进行亚组分析。结果外周血T、B和NK细胞水平在化疗2周后均明显降低(P<0.01),NK细胞和CD8^+T细胞在3月内可恢复至化疗前水平,但CD4^+T细胞和B细胞在化疗后9月后仍不能恢复至化疗前水平(P<0.05);采用含多西他赛方案的患者和使用其他方案的患者相比,淋巴细胞恢复的时间明显减慢(P<0.05)。结论化疗对非小细胞肺癌患者的免疫系统可能存在长期影响,在制定化疗方案时需要结合多方面因素综合考虑。
Objective To investigate change of lymphocyte subsets before and after chemotherapy in patients with NSCLC,to explore the inlfuences of current regimens and clinic-pathological factors on circulating lymphocyte levels and phenotypes. Methods We used lfow-cytometry to assess circulating lymphocyte levels and phenotypes in 87 NSCLC patients before chemotherapy and at time-points from 2 weeks to 9 months after chemotherapy completion. Results Levels of B,T and NK cells were signiifcantly reduced 2 weeks after chemotherapy （P〈0.01）, levels of all cells recovered to some extent, although B and CD4＋ T cells remained signiifcantly depleted even 9 months post chemotherapy differences in chemotherapy regimen were associated with significant differences in depletion extent or repopulation dynamics （P〈0.05）. Conclusion Chemotherapy of NSCLC is associated with long-term changes in immune parameters that should be considered during clinical management.
出处
《中国继续医学教育》
2016年第21期29-31,共3页
China Continuing Medical Education
