川崎病11β-羟基类固醇脱氢酶与促炎性细胞因子的变化及意义

Significance of 11β-Hydroxysteroid Dehydrogenase and Proinflammatory Cytokines in Kawasaki Disease

目的:探讨11β-羟基类固醇脱氢酶(11β-HSD)与促炎性细胞因子在川崎病(KD)发生、发展中的变化及意义。方法:应用Real-time PCR检测KD患儿急性期及治疗后外周血单核细胞内11β-HSD mRNA的表达,应用免疫印迹方法检测11β-HSD蛋白的表达;应用酶联免疫吸附试验检测血浆IL-17A和IL-6水平。结果:病例组患儿治疗前外周血单核细胞内11β-HSD1 mRNA表达水平及IL-17A和IL-6水平分别为7.13±0.79、(43.40±5.20)pg/m L、(68.30±6.26)pg/m L,较正常对照组1.00±0.07、(24.30±2.26)pg/m L、(30.04±2.86)pg/m L明显升高(P<0.01),而治疗后结果分别为3.43±0.52、(27.30±2.50)pg/m L、(38.30±3.50)pg/m L,均较治疗前明显下降(P<0.01);治疗前11β-HSD2 mRNA表达水平为0.32±0.05,明显低于正常对照组1.00±0.06(P<0.01),治疗后水平为0.82±0.04,较治疗前明显升高(P<0.01)。免疫印迹检测11β-HSD蛋白表达水平的结果分析与11β-HSD mRNA表达水平结果分析一致。结论:KD急性期11β-HSD、促炎性细胞因子在调节KD炎症反应中发挥着重要作用。

Objective： To investigate the changes and significance of 11β-hydroxysteroid dehydrogenase（ 11β-HSD） and proinflammatory cytokines in the occurrence and development of Kawasaki disease（ KD）. Methods： Real-time PCR was performed to detect the mRNA expressions of 11β-HSD in peripheral blood monocytes in acute phase and after treatment. Western blot was performed to detect the protein expressions of 11β-HSD. ELISA was performed to detect the levels of IL-6 and IL-17 in plasma. Results： The expression levels of 11β-HSD1 mRNA,IL-17 A and IL-6 in acute phase were 7.13± 0.79,（ 43.40± 5.20） pg / m L,（ 68.30± 6.26） pg / m L,significantly higher than the normal levels 1. 00 ± 0. 07,（ 24. 30 ± 2. 26） pg / m L,（ 30. 04 ± 2. 86） pg / m L（ P〈0. 01）,after treatment the levels were 3. 43 ± 0. 52,（ 27. 30 ± 2. 50） pg / m L,（ 38. 30 ± 3. 50） pg / m L,which were significantly reduced than before（ P〈0.05）. The expression level of 11β-HSD2 mRNA was 0.32± 0.05 before treatment,lower than the normal group（ P〈0.01）,while significantly increased after treatment than before（ P〈0.01）. The results of Western blot were consistent with those of Real-time PCR. Conclusion： 11β-HSD and the level of proinflammatory cytokines in acute phase of KD are important factors in regulating inflammatory responses.