DNA保护银纳米团簇的合成和应用

Synthesis and Applications of DNA Protected Silver Nanoclusters

银纳米团簇因其独特的与尺寸相关的光、电、磁和催化性能,引起了相关研究人员的高度关注,我们团队一直专注于研究用基于DNA保护的银纳米团簇监测DNA、Hg2+和巯基化合物。发现发生在DNA/银纳米复合物与G-四链体/血红素之间光诱导电子转移(PET),伴随着DNA/银纳米荧光减弱。这一新的PET系统使目标生物分子,如DNA和敏感性高的ATP获得特异性和多样性的检测。首次提出一种以DNA单体作为支架的高产率银纳米簇的合成方法。在这项研究中,采用密度泛函计算理论解释了DNA保护的银纳米团簇的形成机理以及为什么富胞嘧啶DNA是荧光银纳米簇的良好支架。研究结果对DNA保护荧光银纳米簇进一步实验和理论研究提供了基本指导思想,最终可能有助于程序化合成具有光致发光性能的DNA稳定银纳米团簇。

Silver nanoclusters have attracted much research interest because of their unique size-dependent optical,electronic,magnetic,and catalytic properties.Our group has focused on the detection of DNA,Hg2＋and biothiols based on DNA protected silver nanoclusters.We have reported photoinduced electron transfer（PET）between DNA/Ag NCs and G-quadruplex/hemin complexes,accompanied by a decrease in the fluorescence of DNA/Ag NCs.The novel PET system enabled the specific and versatile detection of target biomolecules such as DNA and ATP with high sensitivity.Recently,we have developed a high-yield synthesis of silver nanoclusters using DNA monomers as the scaffolds for the first time.In the study,we have explained the mechanism of the formation of DNA-protected silver nanoclusters and the reason why cytosine-rich DNA strands are good scaffolds for fluorescent silver nanoclusters by using density-functional computations.Our results provide basic guidelines for further experimental and theoretical studies on DNA-protected fluorescent silver nanoclusters and may ultimately contribute to the programmed synthesis of DNA-stabilized silver nanoclusters with photoluminescence properties.