人端粒酶反转录酶mRNA在不同类型黑素瘤中的表达及其意义

Expression and significance of human telomerase reverse transcriptase mRNA in different subtypes of melanoma

目的：检测黑素瘤组织中人端粒酶反转录酶（hTERT）mRNA的表达，并分析其表达与黑素瘤类型及患者临床病理特征之间的关系。方法采用实时荧光定量聚合酶链反应（PCR）法检测64例黑素瘤组织和30例色素痣组织中hTERT mRNA表达水平；采用SPSS 17.0软件分析黑素瘤hTERT mRNA的表达与临床病理特征间的关系。结果黑素瘤组织中hTERT mRNA相对表达水平为52.43±5.42，高于色素痣组织的21.38±3.73，差异有统计学意义（t＝4.72，P＝0.000）。hTERT mRNA的表达与黑素瘤患者的年龄、性别、民族均无关（均P＞0.05），而与黑素瘤类型、淋巴结转移、Clark分级相关（均P＜0.05）。黏膜黑素瘤中hTERT mRNA表达水平高于肢端、非肢端黑素瘤（t＝7.71，P＝0.001），而肢端、非肢端黑素瘤间差异无统计学意义（P＞0.05）。结论黑素瘤组织中hTERT mRNA的表达升高，其中以黏膜黑素瘤尤为显著。 hTERT可能与黑素瘤的发生、发展相关。

Objective To detect the expression of human telomerase reverse transcriptase （hTERT） mRNA in the melanoma, and to analyze the relationship between the expression and subtypes and clinicopathological features of melanoma. Methods Expression of hTERT mRNA was detected by real-time quantitative PCR in 64 cases of melanoma and 30 cases of nevus. SPSS 17.0 software was used to analyze the relationship between hTERT mRNA expression and clinical pathological features of melanoma. Results The relative expression of hTERT mRNA in melanoma tissues was higher than that in nevus tissues [（52.43±5.42） vs （21.38±3.73）, t= 4.72, P= 0.000]. The expression of hTERT mRNA in melanoma had no significant correlation with age, gender, ethnicity （all P〉 0.05）, but had relationship with subtypes, lymph node metastasis, Clark classification （all P〈 0.05）. The expression of hTERT mRNA in mucosal melanoma was significantly higher than that of acral and non-acral melanoma （t= 7.71, P= 0.001）, while the expression of acral and non-acral melanoma had no difference （P〉 0.05）. Conclusions The expression of hTERT mRNA in melanoma is high, especially in mucosal melanoma. hTERT may play an important role in the occurrence and development of melanoma.