沙棘品种深秋红茎尖组织培养

Tissue Culture of Stem Tip of Seabuckthorn Variety Shenqiuhong

[目的]筛选沙棘品种深秋红茎尖组织培养基配方。[方法]选用不同培养基,以7月中旬至8月下旬采集的大田茎尖为外植体,对沙棘品种深秋红的组织培养技术进行研究。[结果]最适初代培养基为1/2MS+6-BA 0.5 mg/L,最适愈伤组织诱导培养基为1/2MS+6-BA 0.5 mg/L+IBA 0.5 mg/L,最适继代培养基为1/2MS+6-BA 0.5 mg/L+IBA 0.2 mg/L,最适腋芽诱导培养基为1/2MS+6-BA 0.5 mg/L。[结论]该研究建立了沙棘品种深秋红茎尖组织培养技术,为其产业化生产奠定基础。

Objective] The aim was to screen out culture medium formula for stem tip of seabuckthorn variety Shenqiuhong.[Method] By using different culture mediums, with stem tip collected from middle Jul.to late Aug.as explants, tissue culture technology of seabuckthorn variety Shenqiuhong was studied.[Result] The optimal primary culture medium was 1/2MS＋6-BA 0.5 mg/L, the optimal callus induction culture medium was 1/2MS ＋6-BA 0.5 mg/L ＋IBA 0.5 mg/L, the optimal subculture medium was 1/2MS ＋6-BA 0.5 mg/L ＋IBA 0.2 mg/L, the appropriate axillary bud induction medium was 1/2MS＋6-BA 0.5 mg/L.[Conclusion] The cultivation technique for stem tip of seabuckthorn variety Shenqiuhong is established, which will lay a foundation for industrialized production.