黄芩素抑制铜绿假单胞菌运动能力的体外研究

In vitro investigate of the inhibition of baicalein on Pseudomonas aeruginosa motility

目的探讨最低抑菌浓度(MIC)的黄芩素对铜绿假单胞菌泳动、丛集运动和颤搐运动的影响,为研究其细菌生物膜的影响奠定基础,从而为临床用药提供参考。方法 2015年3月-2015年7月将所做试验组分为空白对照组、不同MIC黄芩素组、克拉霉素组;两倍稀释法测定药物MIC;制成不同培养基,试验菌株隔夜培养,无菌牙签接种于上述培养皿,观察以接种点为中心的细菌运动形状及轨迹,测定运动环评估细菌的运动能力。结果不同MIC黄芩素组形成的泳动同心圆区域直径与空白组比较,差异无统计学意义;黄芩素各MIC组、空白对照组形成的同心圆区域直径大于克拉霉素组(P<0.05),丛集运动能力检测,各组均以接种点为中心,但空白组周边形成齿轮样放射状轨迹,128μg/ml、64μg/ml的黄芩素组及克拉霉素组,其运动区呈以接种点为中心的圆状区域,周边光滑,无伪足样放射,且区域直径<空白对照组(P<0.05)。结论各MIC黄芩素未显示出抑制铜绿假单胞菌泳动的效应,但均可明显抑制铜绿假单胞菌的丛集运动和颤搐运动,64μg/ml黄芩素即显示出抑制铜绿假单胞菌颤搐运动能力,且与克拉霉素抑制铜绿假单胞菌颤搐运动能力相当。

OBJECTIVE To investigate the destructive effect of minimum inhibitory concentration（MIC）of baicalein on swimming motility,swarmming motility and twitching motility of Pseudomonas aeruginosa and provide basis for studying its effects on bacterial biofilm,so as to provide references for clinical medication.METHODS The experiments were divided into control group,different sub-MIC concentrations of baicalein group and clarithromycin group from Mar.2015 to Jul.2015.The MIC of test drugs were measured by the double dilution method.Different medium were made,germ-free toothpick was vaccinated by an overnight single strain in the tablet,the shape of the zone that bacteria was observed,and the zone of motilities were measured to assess the motility of bacteria.RESULTS The concentric region diameters formed by the swimming motility of P.aeruginosain different concentrations of baicalein groups had no difference with control group,and the diameters of different concentrations of baicalein groups and control group were bigger than clarithromycin group（P〈0.05）.The surrounding of the circular area formed a sharp of radial-like gear in control group,but smooth surrounding could be seen in baicalein groups with the concentrations of 128μg/ml,64μg/ml and clarithromycin group,without pseudopodial radiation.Their diameters of the circular area were smaller than those of control group（P〈0.05）.CONCLUSIONIt is shown that sub-MIC concentrations of baicalein cannot inhibit the swimming motility of P.aeruginosa,but can inhibit the swarming motility and twitching motility significantly.It is shown that baicalein with the concentration of64μg/ml can inhibit the twitching motility of P.aeruginosaand have no difference with clarithromycin.