原位杂交法检测人乳头状瘤病毒感染与免疫球蛋白样转路子4水平的相关性分析

Correlation analysis of HPV infection and ILT4level in patients with ovarian cancer detected by in situ hybridization method

目的分析原位杂交法检测卵巢癌患者人乳头状瘤病毒(HPV)感染率与免疫球蛋白样转路子4(ILT4)水平的相关性,为临床卵巢癌的治疗提供参考依据。方法对2012年7月-2014年12月医院189份正常卵巢标本及卵巢上皮良性肿瘤切除标本进行研究,对其中43份正常卵巢组织标本,43份卵巢上皮良性肿瘤(卵巢囊腺瘤)标本、卵巢癌标本52份、交界性肿瘤标本51份等4种标本进行ILT4水平检测,并分析ILT4与卵巢癌中的HPV之间的相关性,数据采用SPSS 18.0软件进行统计分析。结果卵巢癌中HPV16/18感染率与交界性肿瘤比较,差异无统计学意义,但明显高于卵巢囊腺瘤及正常卵巢组织(P<0.05);ILT4阳性表达与HPV16/18感染具有较好的正相关性。结论 ILT4与卵巢癌HPV16/18感染具有正相关性,ILT4可能对卵巢癌中HPV16/18感染产生协同作用。

OBJECTIVE To analyze the correlation of Human papillomavirus（HPV）infection rate and the ILT4 level in patients with ovarian cancer detected by in situ hybridization method,so as to provide reference for clinical treatment of ovarian cancer.METHODS A total of 189 cases of normal ovarian and ovarian benign epithelial tumor resection specimens from Jul.2012 to Dec.2012 were studied,including 43 cases of normal ovarian tissue samples,43 cases of ovarian benign epithelial tumor（ovarian cystadenoma）specimens,52 cases of ovarian cancer specimens,and 51 cases of borderline tumor specimen.ILT4 levels of these four specimens were detected,and the correlation between ILT4 and HPV in ovarian cancer was analyzed using SPSS 18.0.RESULTS The HPV16/18 infection rate of ovarian cancer and that in borderline tumors had no significant difference,but significantly higher than that in ovarian cystadenoma and normal ovarian tissue（P〈0.05）.ILT4 positive expression and HPV16/18 infection had good positive correlation.CONCLUSIONILT4 has a positive correlation with ovarian cancer HPV16/18 infection and may promote HPV16/18 infection of ovarian cancer.