肠道病毒71型湖州分离株的遗传进化和重组分析

Genetic evolution and recombination of enterovirus 71 isolated in Huzhou

目的对湖州市肠道病毒71型(EV71)进行基因特征及重组特点分析。方法对2011-2012年湖州市EV71分离株进行全基因组测序,并与其基因型及A组肠道病毒其他血清型的原型株进行全序列比对,使用Mega 4.0软件绘制系统进化树。然后利用Simplot 3.5.1软件进行相似性分析,对基因重组加以验证。结果 P1区系统进化分析显示,2011-2012年湖州市EV71株属于C4基因型的C4a亚型。P1、P2、P3区的系统进化和相似性分析证实湖州市分离株在非结构蛋白2B编码区存在EV71 C基因型和Cox A16、14、4型间重组。结论 2011-2012年湖州市EV71分离株为C4a亚型,与2004年以来中国大陆优势株流行趋势一致。EV71 C4基因型湖州市株存在型间重组现象。

Objective To understand the genetic evolution and recombination of enterovirus 71（ EV71） isolates in Huzhou during 2011-2012. Methods A total of 2 EV71 isolates were used for whole genome sequencing and the sequences were aligned with other genotypes of EV71 and prototype strains of EV-A. Phylogenetic tree was constructed with software Mega4. 0 and similarity analysis was done with software Simplot 3. 5. 1. The recombination confirmation were performed. Results The phylogenetic analysis based on P1 regions revealed that the EV71 isolated in Huzhou during 2011-2012 belonged to C4 a in genotype C4. Similarity analysis and phylogenetic analysis based on the P1,P2,P3 genomic regions confirmed the recombination between EV71 genotypes C and Cox A16,14 and 4 at region 2B. Conclusion EV71 isolated in Huzhou during 2011-2012 belonged to C4 a in genotype C4,which was the predominant virus genotype circulating in the mainland of China since 2004,and the intratypic recombination was found in EV71 subgenotype C4.