组织因子途径抑制物-2在宫颈鳞癌组织中的表达及其作用

TFPI-2 expression and its role in cervical squamous carcinoma tissues

目的：探讨组织因子途径抑制物-2（TFPI-2）在正常宫颈、宫颈上皮内瘤病变（CIN）和宫颈鳞癌组织中的表达及其作用。方法选取2011年1月至2012年12月在瑞慈医院行宫颈切除的患者114例，其中宫颈鳞癌患者42例，CIN 患者45例，正常宫颈患者27例，采用免疫组化方法检测各宫颈组织 TFPI-2蛋白表达情况。结果宫颈鳞癌组织 TFPI-2表达强度明显低于其他宫颈组织，差异有统计学意义（宫颈癌组织比正常宫颈组织χ2＝59．670，P ＜0．05；宫颈癌组织比 CINⅠχ2＝52．170，P ＜0．05；宫颈癌组织比 CINⅡ／Ⅲχ2＝32．759，P ＜0．05）；CINⅡ／Ⅲ TFPI-2表达程度较正常宫颈组织和 CINⅠ降低，差异有统计学意义（χ2＝33．383，P ＜0．05）；正常宫颈组织与 CINⅠTFPI-2表达程度比较差异无统计学意义（χ2＝4．903，P ＞0．05）。不同分化程度宫颈鳞癌组织 TFPI-2表达比较差异无统计学意义（χ2＝4．633，P ＞0．05）；Figo Ⅰ期宫颈鳞癌组织TFPI-2表达程度明显高于 Figo Ⅱ期宫颈鳞癌组织，差异有统计学意义（χ2＝6．509，P ＜0．05）；无淋巴结转移宫颈鳞癌组织 TFPI-2表达程度明显高于有淋巴结转移宫颈鳞癌组织，差异有统计学意义（χ2＝10．916，P ＜0．05）。结论 TFPI-2表达减少可能参与宫颈鳞癌的发生发展，且 TFPI-2表达下调与宫颈鳞癌的预后有关。

Objective To investigate expression and role of tissue factor pathway inhibitor -2（TFPI -2）in normal cervix,cervical intraepithelial neoplasia（CIN）and cervical squamous cell carcinoma.Methods From Janu-ary 2011 to December 2012,114 patients with cervical resection in our hospital were selected,among them,42 patients with cervical squamous cell carcinoma,27 cases with CIN,45 cases of normal cervix.Immunohistochemical method was used to detect the expression of TFPI -2 protein in cervical tissues.Results The expression of TFPI -2 in cer-vical cancer tissues was significantly lower than that in other cervical tissues,the difference was statistically significant （cervical cancer tissues vs.normal cervical tissues χ2 =59.670,P 〈0.05;cervical cancer tissues vs.CINⅠ χ2 =52.170,P 〈0.05;cervical cancer tissues vs.CINⅡ/Ⅲ χ2 =32.759,P 〈0.05）.The expression of CIN Ⅱ/Ⅲ TFPI-2 was significantly lower than that of normal cervical tissues and CIN Ⅰ,the difference was statistically significant （χ2 =126.78,P 〈0.05）.The expression level of TFPI -2 in normal cervix tissues and CIN Ⅰ had no statistically significant difference（χ2 =4.903,P 〉0.05）.There was no significant difference in TFPI -2 expression in cervical cancer tissues with different differentiation levels（χ2 =4.633,P 〉0.05）.Figo Ⅰcervical cancer TFPI -2 expression was significantly higher than Figo Ⅱ cervical cancer,the difference was statistically significant（χ2 =6.509,P 〈0.05）.The expression of TFPI -2 in cervical cancer tissues without lymph node metastasis was significantly higher than that in cervical cancer tissues with lymph node metastasis,the difference was statistically significant （χ2 =10.916,P 〈0.05）.Conclusion The reduction of TFPI -2 expression may be involved in the development of cervi-cal cancer,and the expression of TFPI -2 is associated with the prognosis of cervical cancer.