肝素对感染性休克犬早期肠黏膜损伤保护作用的研究

Protective effects of heparin on early septic shock of intestinal mucosal injury in dogs

目的通过对血浆标示物D-乳酸和I-FABP的测定及分析，验证感染性休克犬早期肠脂肪酸结合蛋白和D-乳酸表达水平发生变化以及肝素对感染性休克犬早期空肠绒毛微循环改善及肠脂肪酸结合蛋白和D晋L酸表达水平的影响。方法通过静脉注射LPS方法建立比格犬早期感染性休克模型。将40只实验犬随机（随机数字法）分为空白对照组，LPS组，基础治疗组，肝素治疗组。术后稳定1h，即T0点，给LPS造模后1h，即T1点，之后序贯观察5h分别为T2，13，T4，T5，T6点。在相应观察时间点（T1、T3、T6时间点）采集犬静脉血，经1500r／rain，15min离心后取血浆，以EP管分装后置入-80℃保存，通过ELISA法测定肠脂肪酸结合蛋白（I-FABP）和D-乳酸（D—lac）。同时应用OPS观察空肠绒毛微循环的变化。结果肝素组IFABP（21．6±2．3）pg／mL比较LPS组IFABP（100．48±8．42）Pg／mL会明显下降，差异具有统计学意义（P〈0．05）；同时微循环有相应的改善。基础治疗组与LPS组比较未见明显变化，P〉0．05。结论感染性休克早期存在肠黏膜微循环障碍及肠黏膜损伤。肝素对早期感染性休克犬肠黏膜微循环障碍具有改善作用，进而保护肠黏膜屏障功能。

Objective Determination of plasma markers of D-lactic acid and I-FABP and analyzed to verify the early septic shock in dogs and intestinal fatty acid binding protein expression levels of D-lactate changes and improvements to septic shock heparin early jejunal villus microcirculation in dogs and intestinal fatty acid binding protein and the expression of D-lactic acid levels, confirmed the protective effect of heparin on intestinal mucosal injury. Methods Beagles early septic shock by intravenous injection of LPS Methods. The 40 animals were randomly random number divided into control group, LPS group, the basic treatment group, heparin group. Stable after 1 hour, i. e. the point TO, to modeling LPS 1 hour, i. e. at T1, after 5 hours was observed sequential T2, T3, T4, TS, T6 point. Observed at the corresponding time points （ T1, T3, T6 time points） canine blood collected by 1 500 rpm, 15 minutes after centrifugation, the plasma to the EP tube into aliquots stored at -80 ℃, measured by ELISA intestinal fatty acid binding protein （IFABP） and D-lactate （D-lac）. Results heparin group IFABP （21.6 ±2. 3 ） pg / mL LPS comparison group IFABP （ 100.48 ± 8.42） pg / mL will be significantly decreased, with statistical significance, P 〈 0. 05 ; basic treatment group and no significant change in LPS group, P 〉 0. 05, on the basis of adequate volume resuscitation on heparin therapy, I-FABP expression levels can be significantly reduced. Conclusions The presence of septic shock early intestinal mucosal microcirculation and intestinal mucosa. Under the premise of its full capacity of recovery, heparin early septic shock in dogs with intestinal mucosal microcirculation improvement, thereby protecting the intestinal mucosal barrier function.