TNF-a-1031,-238,-308基因多态性与卵巢早衰的关系

Association of TNF-a-1 031,-238,-308 Gene Polymorphisms with Premature Ovarian Failure in Chinese Women

目的探讨肿瘤坏死因子(TNF)-a基因中-238,-1 031,-308位点多态性与卵巢早衰的关系。方法选择200例卵巢早衰妇女和200例对照者作为研究对象,抽取外周血白细胞中的DNA,应用聚合酶链式反应-限制性片段长度多态性技术分析TNF-a基因中-238G/A、-1 031T/C、-308G/A位点多态性,采用卡方检验对不同基因型之间的差异进行比较。结果卵巢早衰组和对照组中TNF-a-238位点和TNF-a-1 031位点的等位基因频率和基因型频率差别无统计学意义(P>0.05)。卵巢早衰组中TNF-a-308位点的G/G基因型频率高于对照组(90%vs 82.5%,P<0.05),且2组间等位基因频率差别有统计学意义(P<0.05)。结论 TNF-a基因中-308位点的单核苷酸多态性可能与卵巢早衰的发生存在相关性。

Objective To investigate the relationships between tumor necrosis factor-alpha （TNF-a） gene-1 031,-238,-308 polymorphisms with premature ovarian failure in Chinese women. Methods Two hundred with premature ovarian failure and two hundred healthy control women were com- pared in the study. TNF-a-1 031, -238, -308 gene polymorphisms were assayed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. All statistical analyses were calculated with SPSS for Windows 16.0. Results There were no significant differences in allele or genotype frequency of TNF-a-238 and TNF-a-1 031 between women with premature ovarian failure and control group（P〉0.05）. The homozygous GG genotype for the SNP-308G/A was significantly higher in patients with premature ovarian failure than in healthy controls （90% vs 82.5%, P〈0. 05）. The allele frequency of the TNF-α- 308G was significantly higher in patients with premature ovarian failure patients than in healthy controls （P〈0.05）. Conclusion The data suggested that the TNF-a-308 gene polymorphisms was associated with premature ovarian failure.