DNA损伤修复基因hMTH 1多态性与尘肺发病风险相关性研究

Relationship between polymorphism of DNA damage repair gene h MTH 1 and risk of pneumoconiosis

目的探究h MTH 1基因型分布与尘肺发病风险的关系,为进一步阐明尘肺的发生发展以及防治尘肺提供新的线索。方法采用病例对照的研究方法,分别收集尘肺组和对照组的个人相关资料,采集外周血样本并提取DNA,利用聚合酶链反应-限制性片段长度多态性分析技术(PCR-RFLP),分析h MTH 1基因内含子4中c.Val 83 Met位点的多态性。结果 h MTH 1基因3种基因型(c.83 Val/Val、Val/Met、Met/Met)在病例组和对照组中的频率分布差异无统计学意义(χ2=0.92,P=0.38)。并且基因型Val/Val和Val/Met+Met/Met(即至少携带一个编码Met的等位基因)在尘肺组和对照组中的分布频率差异无统计学意义。结论 h MTH 1 Va l83 Met多态性与尘肺病的发病风险可能无关。

[Objective]To explore the relationship between the h MTH 1 polymorphism and the risk of pneumoconiosis,provide new clues for the occurrence,prevention and treatment of pneumoconiosis. [Methods]Case-control study was used to collect personal related information of the case group and the control group. Peripheral blood samples were collected to extract DNA and polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method was used to detect the h MT H 1geneintron 4 c.Val83 Met polymorphisms. [Results]There was no statistically significant difference in the distribution of h MTH1 gene Val/Val,Val/Met and Met/Met genotypes between the case group and the control group（χ2=0.92,P=0.38）. And there was no statistically significant difference in the distribution of genotype Val/Val and genotype Val/Met ＋Met/Met（allele carrying at least one Met） between the case group and the control group. [Conclusion] No relationship is found between h MT H 1polymorphism and the risk of pneumoconiosis.