苯和甲醛吸入染毒对小鼠血清、肺和肝脏中SOD、GSH-Px和MDA影响

Effect of benzene and formaldehyde on SOD,GSH-Px and MDA in serum,lung and liver in mice by inhalation exposure

目的了解苯和甲醛吸入染毒对小鼠血清、肺、肝脏的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)的活力影响。方法将昆明种雄性小鼠40只,随机分为4组,分别为对照组、甲醛组(3 mg/m3)、苯组(2 000 mg/m3)和联合组(甲醛3 mg/m3+苯2 000 mg/m3)。每天给予4 h吸入染毒,每周5 d,连续5周。测定动物体重、肺脏和肝脏的脏器系数;测定血清、肺、肝脏的SOD、GSH-Px和MDA水平。结果经过5周染毒,苯组和联合染毒组动物平均体重低于对照组(P<0.05),3个组动物的血清中SOD水平明显低于对照组(P<0.01),苯组和联合染毒组肺脏系数明显低于对照组(P<0.01),苯组和联合组肺脏SOD、GSH-Px水平高于对照组(P<0.05),联合组的肺脏MDA水平较对照组明显升高(P<0.01);3个染毒组的肝脏系数均明显低于对照组(P<0.01),苯组和联合组肝脏SOD和MDA水平高于对照组(P<0.01),甲醛组和联合组的GSH-Px的水平高于对照组(P<0.01)。结论苯和甲醛吸入后可能造成血清中SOD水平下降,引起肺、肝脏的SOD和GSH-Px的活力增高,苯和甲醛的联合染毒造成的过氧化损伤大于单独染毒。

[Objective]To analyze the effect of benzene and formaldehyde on activities of SOD,GSH-Px and MDA in serum,lung and liver in mice by inhalation exposure. [Methods]40 male Kunming mice were randomly divided into 4 groups,including the control group,the formaldehyde group（3 mg/m3）,the benzene group（2 000 mg/m3）and the combine exposed group（3 mg/m3 formaldehyde and 2 000 mg/m3benzene）. The animals were treated with inhalation exposure 4 hours a day,5 days a week for 5weeks. The body weight of animals,the coefficient of lung and the coefficient of liver,as well as the levels of SOD,GSH-Px and MDA in of serum,lung and liver were tested. [Results]After the exposure for 5weeks,the body weight of the benzene group and the combine exposed group was lower than that of the control group（P〈0.05）,the serum SOD level in three exposure groups was significantly lower than that in the control group（P〈0.01）,the lung coefficient in the benzene group and the combine exposed group was significantly lower than that in the control group（P 0.01）,the levels of SOD and GSH-Px in lung of the benzene group and the combine exposed group were significantly higher than those of the e control group（P〈0.05）,the MDA level in lung of the combine exposed group was significantly higher than that of the control group（P〈0.01）,the liver coefficient in three exposure groups was significantly lower than that in the control group（P〈0.01）,the levels of SOD and MDA in liver of the benzene group and the combine exposed group were significantly higher than those of the e control group（P〈0.01）,and the level GSH-Px level in the formaldehyde group and the combine exposed group were was significantly higher than that in the control group（P〈0.01）. [Conclusion]The inhalation exposure of benzene and formaldehyde could reduce the activity of SOD in serum,and enhance the activities of SOD and GSH-Px in lung and liver. The combined exposure to benzene and formaldehyde has severer oxidative damage than the single exposure.