海河天津城市区域人类腹泻病毒的污染特征调查

Contamination characteristics of human diarrhea virus in Tianjin reach of Haihe river

目的研究城市典型河流生境条件下人类腹泻病毒的污染特征,阐明环境水体病毒污染规律。方法于2014年3月—2015年2月,采集海河天津城市区域水样,采用实时荧光定量PCR技术检测腹泻病毒[轮状病毒(HRVs)、诺如病毒(HuNoVs)、肠道腺病毒(HadVs)、星状病毒(AstVs)、肠道病毒(EnVs)]的浓度。结果在36份海河水样中,肠道腺病毒和星状病毒的检出率最高,均为91.7%,其次是肠道病毒83.3%,诺如病毒75.0%,而轮状病毒的检出率最低,为58.3%。肠道病毒在7—9月出现高峰期,其他腹泻病毒均在10月—次年1月出现高峰期。结论人类腹泻病毒在海河中均呈现一定的时间分布规律且具有明显的季节性。

Objective To understand the contamination characteristics of human diarrhea virus under the urban typical river habitat conditions in Tianjin reach of the Haihe river. Methods A total of 36 water samples were collected during March 2014 to February 2014 from Tianjin reach of the Haihe river to monitor the human diarrhea virus levels of HRVs,HuNoVs,HAdVs,AstVs and EnVs. Results In the 36 Water samples,both of HAd Vs and Ast Vs showed the highest detection rate of 91.7%,following EnVs of 83.3%,HuNoVs of 75.0%,and HRVs showed the lowest detection rate. EnVs reached the peak level in June2014 to August 2014,other diarrhea viruses were in the peak period of October 2014 to January 2015. Conclusion Human diarrhea virus contamination in Tianjin reach of the Haihe river shows an obvious seasonal distribution.