电针对大鼠急性骨骼肌损伤修复中生长相关蛋白和聚集蛋白表达的影响

Effects of electroacupuncture on expressions of growth associated protein-43 and agrin in acute skeletal muscle injury

目的:通过观察电针治疗对急性骨骼肌损伤中生长相关蛋白(GAP-43)及聚集蛋白(agrin)表达的影响,探讨电针促进受损骨骼肌功能恢复的作用机制。方法:将32只SD大鼠随机分为正常组(A组,n=4)、模型组(B组,n=4)、自然恢复组(C组,n=12)、电针组(D组,n=12),A组为空白对照不做任何处理,其余各组使用自制重物打击器建立骨骼肌急性钝挫伤模型,C组不做电针处理自然恢复,D组于造模后48h开始电针干预,每日1次,每次15min。于造模后24h处死B组取材观察造模是否成功,C组及D组于建模后第7、14、21天三个时间点同时取材使用HE染色观察组织形态变化,Western-bolt检测GAP-43及agrin表达情况。结果:HE染色显示:与A组比较,各组肌细胞大量溶解、肌纤维排列紊乱及炎性细胞浸润。D组与C组比较,肌卫星细胞增殖较快,新生肌纤维明显增多,修复更为迅速。在正常组织中GAP-43表达量极少,骨骼肌损伤后各组中GAP-43表达量显著增高(P<0.05);在第14天,GAP-43表达量达到高峰,D组继续表达呈上升趋势(P<0.01),C组开始下降,但仍高于A组(P<0.05)。与A组相比,骨骼肌受损后各时间点C、D两组agrin的表达明显增多(P<0.05),其中D组差异最为显著(P<0.01)。结论:电针有促进受损骨骼肌恢复的作用,可能与提高GAP-43和agrin的表达水平有关。

Objective： To observe effects of electroacupuncture on agrin in acute injury of skeletal muscle, to investigate of damaged skeletal muscle function. of expressions growth associated protein （GAP-43） and effects of electroacupuncture on mechanism of recovery Method： Thirty-two SD rats were randomly divided into normal group （group A, n=4）,model group （group B, n=4）, natural recovery group （group C, n=12）, electroacupuncture group （group D, n=12）.The group A was a control group without any treatment, while other groups were established acute skeletal muscle blunt contusion model, by using homemade heavy blow group C without electroacupuncture treatment, only had natural recov- ery, group D after modeling 48h had electroacupuncture, 1 times a day, every time 15min. At 24h after model- ing, rats group B were sacrificed and observed. After modeling, at the 7^th, 14^th,21^st d three time points group C and group D were used to observe the morphological changes with HE staining, and defect GAP-43 as well as agrin expressions with Western-blot technique. Result：HE staining showed that, compared with group A, the muscle cells of other groups dissolved, muscle fi- bers arranged in disorder and inflammatory cell infiltrated. Compared with group C, the muscle satellite cells of group D increased rapidly, new muscle fibers increased significantly, and repaired more rapidly. The expres- sion of GAP-43 in normal tissues was few, but in bone injury increased significantly（P〈0.05）. At the 14^th d, the expression of GAP-43 reached the peak, but in group D the expression increased （P〈0.01）, and in group C the expression began to decline, but still higher than that of group A （P〈0.05）.Compared with group A,the agrin expression in skeletal muscle damage increased significantly in groups C and D（P〈0.05）, the difference at three time points in D group was the most significant （P〈0.01）. Conclusion： Electroacupuncture could promote the role of skeletal muscle function recovery. It may be related to the increasing of GAP-43 and the expression of agrin.