加味苓桂术甘方对酒精性脂肪肝防治作用的实验研究

Linggui Zhugan decoction on prevention and treatment of alcoholic fatty liver

目的:研究加味苓桂术甘方对酒精性脂肪肝防治作用。方法:SD雄性大鼠60只,随机分为6组,每组10只。50只给予15%乙醇灌胃,同时饲喂高脂饲料,制备酒精性脂肪肝大鼠模型。模型组动物给予生理盐水灌胃,实验组动物给予不同剂量的加味苓桂术甘汤和乙醇灌胃。通过观察和分析大鼠一般情况、肝脏指数、肝脏组织病理形态、血清肝功能和血脂含量等指标,研究加味苓桂术甘方对酒精性脂肪肝防治效果。结果:与模型组比较,加味苓桂术甘方干预酒精性脂肪肝动物模型后,行为有明显改善。病理分析显示,加味苓桂术甘方中剂量和大剂量组肝脏脂肪变性和炎症明显减轻。血清肝功能检测结果显示,加味苓桂术甘方中剂量和大剂量组动物用药后血清ALT、AST、TG、TC和FFA水平均明显下降。结论:加味苓桂术甘方对酒精性脂肪肝具有显著的防治作用。

Objective ：To examine the effect of Linggui Zhugan decoction on prevention and treatment of alcoholic fatty liver .Methods ：60 male Spague-Dawlayrats were randomly divided into 6 groups with 10 rats in each group .50 rats were fed with high fat diet and ethanol fed to establish the experimental model of alcoholic fatty liver .The animal model group were administered saline ,and the animals of experimental group were treated with different doses of Linggui Zhugan and ethanol gavage .General conditition ,liver index ,liver pathology ,hepatic function and blood lipid contents of rats were to observed and analysied to investigate the effect of Linggui Zhugan decoction on prevention and treatment of alcoholic fatty liver .Results ：Compared with the model group ,behaviors of animals of nonalcoholic fatty liv-er disease model group treated with Linggui Zhugan decoction have significantly improved .Pathological analysis showed that the liver steatosis and inflammation of animalstreated with Linggui Zhugan decoction at large dosage and medium dosage significantly reduced .Serum liver function test results show that ALT ,AST of serum in groups of animals treated with Linggui Zhugan decoction at large dosage and medium dosage were significantly decreased .Also TG ,TC and FFA levels were significantly decreased in groups of animals treated with Linggui Zhugan decoction at large dosage and medi-um dosage .Conclusion ：Linggui Zhugan decoction has significant preventive effect on alcoholic fatty liver .