落花生枝叶水煎剂提升SOD作用对高蛋氨酸灌胃大鼠血管内皮的保护作用

Peanut leaves decoction protective effects of SOD to enhance the effect of the high methionine fed rats vascular endothelium

目的:初步探讨落花生枝叶水煎剂对高蛋氨酸灌胃大鼠干预作用及量效关系。方法:设ZC、MX、XY和ZY、ZD组,前6周除ZC组给予蒸馏水灌胃外其它四组通过灌胃给予L-蛋氨酸制作高蛋氨酸饮食模型。6周后在此基础上,灌胃给药干预4周,取材,检测血清SOD含量、胸主动脉组织结构和IL-8、ICAM-1mRNA表达。结果:与ZC组比较,MX组血清SOD值明显降低,西药组、中药组、中药大剂量组较正常组、模型组均有所提高(P<0.05)。PCR结果提示高浓度的落花生枝叶水煎剂可以抑制细胞间黏附因子ICAM-1表达;免疫组化提示落花生枝叶水煎剂可抑制白介素-8(IL-8)的表达。结论 :落花生枝叶水煎剂具有提升SOD的作用,并且对高蛋氨酸灌胃大鼠血管内皮损伤具有保护作用,作用与辛伐他汀相似。

Objective ：To investigate the peanut leaves decoction on rats fed high methionine intervention and dose-effect relationship .Method ：Let ZC ,MX ,XY and ZY ,ZD group ,in addition to six weeks before foreign ZC group were given distilled water orally by intragastric administration of the other four groups L- methionine production of high methionine diet model .After six weeks on the basis of gavage intervention for 4 weeks ,subjects ,serum SOD content , structure and thoracic aorta IL-8 ,ICAM-1mRNA expression .Results ：Compared with ZC group ,MX serum SOD was significantly lower ,western medicine , traditional Chinese medicine group , TCM high dose group than in the normal group ,the model group were higher （P〈 0 .05） .PCR results suggest that high concentrations of groundnut leaves decoc-tion can inhibit intercellular adhesion molecule ICAM-1 expression ;immunohis to chemistry tips groundnut leaves decoc-tion can inhibit the expression of interleukin -8 （IL-8） .Conclusion ：Peanut leaves decoction has elevated the role of SOD , and fed high methionine vascular endothelial injury in rats ,and the effect is similar to simvastatin .