鸭坦布苏病毒在感染蛋鸭卵巢、脾脏及泄殖腔的分布规律

Study on Distribution of DTMUV in Ovaries,Spleen and Cloaca of Layer Ducks

探讨鸭坦布苏病毒在感染蛋鸭卵巢、脾脏及泄殖腔分布规律,为疫苗研究和筛选提供参考依据,选用210只200日龄~220日龄无鸭坦布苏病毒感染的蛋鸭,随机分为7组,1组~6组腿部肌肉注射鸭坦布苏病毒液,7组腿部肌肉注射生理盐水作为对照组,攻毒后每天观察临床症状,分别于感染后第2、5、8、11、16、21天分6批次处死,取脾脏、卵巢及泄殖腔进行病毒分布情况检测。结果显示,攻毒后蛋鸭采食量、产蛋率均降低,并排有绿色粪便;剖检发现脾脏充血、肿大,卵巢萎缩,卵泡破裂,个别出现卵黄性腹膜炎;通过RT-PCR检测不同时间段卵巢、脾脏、泄殖腔等带排毒情况,并结合卵巢病变情况,可知本次试验中不同感染剂量的鸭坦布苏病毒在卵巢和脾脏内分别在第11天和第5天带毒最多,为今后疫苗的研制及免疫效果判定提供了依据。

This study mainly discussed the distribution regulation of DTMUV in ovary, spleen and cloaca of infected layer ducks for the reference basis of vaccine research and screening.This study selected 210 layer ducks with the age of 200-220 days and non-infected DTMUV were randomly divided into 7 groups, 1-6 groups with intramuscular injection of DTMUV,control group with normal saline. The clinical symptoms were observed after challenge. After infection in 2, 5,8,11,16,21 days, the ducks were killed and the spleen, ovary and cloaca were collected.The results of this experiment revealed that layer ducks were lower in the intakes and laying rates after challenge.The autopsy found that spleen hyperemia, swelling, shrunken oaries, ruptured follicle, and yolk peritonitis occurred.RT-PCR was used to detect the virus contents in ovaries, spleens and cloaca of infected ducks in different time, and combined with ovarian lesions.The results showed that DTMUV contents in the ovaries and spleen were the most in llth day and 5th day. The study laid the foundation for the future development and immune effect determination of vaccines.