热休克蛋白70对HBV复制的影响

Effect of HSP70 on HBV replication

目的:探讨过表达热休克蛋白70(HSP70)对乙型肝炎病毒(HBV)复制的影响。方法:运用RTq PCR检测HBV复制对HSP70表达的影响及过表达HSP70对HBV 3.5 kb m RNA的影响;运用Western blot法验证HSP70过表达效果及过表达HSP70对HBV核心蛋白的影响;RT-q PCR和Southern blot法分析过表达HSP70对HBV复制中间体的影响;运用双萤光素酶报告系统检测过表达HSP70对HBV启动子活性的影响。结果:HBV复制显著抑制HSP70的m RNA水平。过表达HSP70抑制HBV复制中间体、3.5 kb m RNA以及核心蛋白的表达,同时抑制HBV核心启动子的活性。结论:HBV复制抑制HSP70的表达。过表达HSP70抑制HBV的复制。本研究结果表明,HSP70通过抑制核心启动子的活性抑制HBV的复制。

AIM： To investigate the role of heat shock protein 70（HSP70）in hepatitis B virus（HBV） replication. METHODS： The effect of HBV replication on the expression of HSP70 was analyzed by RT-q PCR. The overexpression efficiency of HSP70 was confirmed by Western blot. The effect of HSP70 overexpression on HBV DNA replicative intermediates was analyzed by RT-q PCR and Southern blot. The effects of HSP70 overexpression on the expression level of HBV 3. 5 kb m RNA and HBV core protein were measured by RT-q PCR and Western blot,respectively. The Effect of HSP70 overexpression on HBV promoter activity was detected by dual luciferase reporter system. RESULTS： The m RNA levels of HSP70 were inhibited by HBV replication. Overexpression of HSP70 repressed the expression of HBV DNA replicative intermediates,3. 5 kb m RNA and core protein,as well as HBV core promoter activity. CONCLUSION： HBV replication inhibits the expression of HSP70. Overexpression of HSP70 represses HBV replication. These data suggest that HSP70 repressed HBV replication by inhibiting HBV core promoter activity.