经导管射频消融去肾交感神经术对犬肾脏水通道蛋白的影响

Effect of renal denervation by radiofrequency catheter ablation on expression of aquaporins in dog kidneys

目的：研究经导管射频消融去肾交感神经术（renal denervation,RDN）对肾脏水通道蛋白（aquaporins,AQP）的影响。方法：选用昆明犬12只,随机分为对照组和消融组,每组6只。消融组采用射频导管消融双侧肾动脉,对照组仅将射频导管送达肾动脉但不消融。术后1月,检测2组犬肾脏去甲肾上腺素浓度、肾脏皮质和髓质AQP水平,以及血压的变化。结果：术后1月,消融组肾脏去甲肾上腺素浓度明显低于对照组（P〈0.01）,消融组肾脏皮质和髓质AQP1~3的表达均低于对照组。相比术前,术后收缩压和舒张压也出现明显下降（P〈0.05）。结论：RDN能降低犬肾脏交感神经活性,并抑制肾脏AQP的表达,这或许是RDN降血压的机制之一。

AIM： To investigate the effect of renal denervation（RDN） by radiofrequency catheter ablation on the expression of aquaporins（ AQP） in dog kidneys. METHODS： Adult Chinese Kunming dogs（ n = 12） were randomly divided into RDN group and control group（6 for each group）. The dogs in RDN group underwent bilateral RDN using radiofrequency catheter ablation,and radiofrequency catheter was positioned in bilateral renal artery without ablation in control group. The levels of norepinephrine（ NE） and AQP1 ~ 3 in the renal tissues were detected 1 month after RDN,and blood pressure（ BP） measurements were performed at baseline and 1 month after RDN. RESULTS： The level of NE in RDN group was significantly lower than that in control group（ P 0. 01）. The expression of AQP1 ~ 3 in the renal cortex and medulla was lower in RDN group than that in control group. RDN also caused a substantial BP reduction（ P 0. 05）.CONCLUSION： RDN substantially decreases the tissue levels of NE and AQP in dog kidneys,and also decreases BP significantly,which might be involved in the mechanism of BP reduction by RDN. Renal sympathetic nerve plays an excitatory role in the regulation of AQP in the kidney.