Tn抗原在宫颈癌组织中的表达及临床意义

Expression and Clinical Significance of Tn Antigen in Cervical Carcinoma

目的:探讨Tn抗原在宫颈癌组织中的发生、发展中的作用,为宫颈癌的早期诊断、治疗提供一定的参考依据。方法:选择2013—2015年吉林大学第二医院的102例临床样本,其中正常宫颈组织14例,宫颈上皮内瘤变(CIN)组织22例,宫颈癌组织66例,采用免疫组织化学方法以半乳糖胺特异性凝集素蚕豆凝集素(vicia villosa lectin,VVL)作为抗体检测不同宫颈组织中Tn抗原表达情况。结果:Tn抗原极少表达于正常宫颈鳞状上皮(7.1%),在CIN和宫颈鳞癌中表达率分别为45.5%和68.8%,而在宫颈腺癌中表达率高达94.1%。在宫颈鳞癌中,Tn抗原的表达与是否存在淋巴结转移有关(r=0.363,P<0.05)。在宫颈腺癌中,Tn抗原表达与肌层浸润深度有关(r=0.361,P<0.05)。结论:Tn抗原可能作为宫颈腺癌的一种肿瘤标志物,有助于宫颈腺癌的筛查诊断,并可能更早地提示宫颈鳞癌淋巴结转移的潜在风险。

Objective To investigate the expression of Tn antigen in cervical carcinoma. To explore the role of Tn antigenin the occurrence and development of cervical carcinoma, and to provide a reference for the early diagnosis, treatment of cervicalcarcinoma. Methods： We screened 102 cervical samples from the Second Hospital of Jilin University from 2013 to 2015,including 66 invasive cervical carcinomas, 22 cervical intraepithelial neoplasia （CIN）, 14 normal cervical squamous epithelia,by immunohistochemistry using biotinylated vicia villosa lectin （VVL）. Results： The Tn antigen was rarely observed in normalsquamous epithelium （7.1%）, and was detected in 45.5% of CIN and in 68.8% of invasive squamous carcinoma, and was highlyobserved in 94.1% of invasive adenocarcinoma. In invasive squamous carcinoma, expression of Tn antigen was closely related tolymph node metastasis （r=0.363,P〈0.05）. In adenocarcinoma, the correlation was shown between Tn expression and depth ofinvation （r=0.361 ,P〈0.05）. Conclusions ：Tn antigen could be used as a marker for cervical adenocarcinoma, and Tn antigencould indicate the potent risk of lymph-nodes metastasis in squamous carcinoma.