不同品系栝楼果皮腺嘌呤和鸟苷含量比较

Comparison of Contents of Adenine and Guanosine in Peels of Three Strains of Trichosanthes kirilowii Maxim

目的比较不同品系栝楼果皮腺嘌呤、鸟苷含量,为选育良种和评价瓜蒌皮药材质量提供依据。方法采用高效液相色谱法测定3个品系栝楼果皮中腺嘌呤、鸟苷的含量,色谱柱为Waters Atlantis T3-C18(4.6 mm×150.0 mm,5μm),流动相为甲醇-水,检测波长254 nm。结果品系Ⅰ、Ⅱ、Ⅲ栝楼果皮腺嘌呤含量分别为0.159 4、0.159 6、0.134 1 mg/g,鸟苷含量分别为0.097 1、0.127 9、0.093 4 mg/g。结论不同品系栝楼果皮鸟苷含量存在较大差异,以品系Ⅱ果皮中的腺嘌呤、鸟苷含量较高,提示选育良种是提高瓜蒌皮药材腺嘌呤、鸟苷含量的有效途径。

Objective To compare the contents of guanosine and adenine in peels of three strains of Trichosanthes kirilowii Maxim.; To provide references for germplasm resources evaluation and breeding of the Chinese medicinal materials. Methods HPLC method was used to determine the contents of guanosine and adenine with Waters Atlantis T3-C18 column（4.6 mm×150.0 mm, 5 μm）. The mobile phase was methanol and water, and the detection wavelength was 254 nm. Results The contents of guanosinein in peels of three strains of Trichosanthes kirilowii Maxim. were 0.159 4, 0.159 6, 0.134 1 mg/g, respectively; the contents of adenine were 0.097 1, 0.127 9, 0.093 4 mg/g, respectively. Conclusion There were significient differences in the contents of adenine among three strains of Trichosanthes kirilowii Maxim.. Strain Ⅱ is with higher level of guanosine and adenine, which implies that the breeding of the Chinese medicinal materals is one way to improve the quality of Trichosanthis Exocarpium.