长链非编码RNA FENDRR对非小细胞肺癌细胞增殖及凋亡的影响

The influence of long non-coding RNA FENDRR to cell proliferation and apoptosis in non-small cell lung cancer

目的:检测长链非编码RNA FENDRR基因在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达及其对A549细胞增殖及凋亡的影响。方法:Real-time PCR检测FENDRR基因在NSCLC组织中的表达;构建FENDRR基因表达载体pc DNA-FENDRR并转染A549细胞,Real-time PCR检测转染效果;MTT法检测A549细胞的增殖活力;流式细胞术测定A549细胞的凋亡率。结果:FENDRR基因在NSCLC组织中的表达显著下调,为癌旁正常组织中FENDRR的37.24%(P<0.01),FENDRR的低表达与NSCLC的低分化及高T分期相关。pc DNA-FENDRR的转染能够显著上调A549细胞中FENDRR mRNA的表达(P<0.01),而FENDRR过表达能够显著抑制A5 4 9细胞的增殖活力,并诱导凋亡由3.41%升高至8.47%(P<0.01)。结论:FENDRR基因的表达下调与NSCLC的发生和进展相关,其在NSCLC中发挥肿瘤抑制基因的作用,抑制细胞增殖并诱导细胞凋亡。

Objective：To detect the expression of long non-coding RNA FENDRR in non-small cell lung cancer（ NSCLC） tissue and the influence of FENDRR gene to cell proliferation and apoptosis of A549 cells.Methods：Real-time PCR was used to detect the expression of FENDRR gene in NSCLC tissues.The transfection with pc DNA-FENDRR up-regulated the expression of FENDRR gene in A549 cells,and the transcription effect was detected by Real-time PCR.MTT and flow cytometry were used to detect cell proliferation and apoptosis of the A549 cells with FENDRR enhancement.Results：FENDRR mRNA low-expressed obviously in NSCLC,was 37.24% of FENDRR in the tumor-adjacent tissues.Low expression of FENDRR was correlated with poor differentiation and high T stages of NSCLC.The transfection of FENDRR expression vector could effectually up-regulate the expression of FENDRR in A549 cells.The FENDRR enhancement could inhibit the proliferation ability and advance cell apoptosis from 3.41%to 8.47% in A549 cells.Conclusion：LncRNA FENDRR low-expresses in NSCLC and acts as a tumor suppressor gene to inhibit the proliferation ability and advance cell apoptosis of NSCLC cells.