次黄嘌呤对营养性肥胖小鼠的抗肥胖作用

Anti-Obesity Effect of Hypoxanthine on Nutritionally Obese Mice

目的:研究次黄嘌呤(hypoxanthine,Hx)对营养性肥胖小鼠抗肥胖作用的影响。方法:将ICR(Institute of Cancer Research)小鼠随机分成6组:空白组、模型组、阳性对照组(左旋肉碱100 mg/kg)和Hx干预组(30、60、90 mg/kg),采用高脂饲料饲喂建立肥胖小鼠模型。口服给药4周后,处死小鼠,收集血液,离心。检测各组小鼠血清甘油三酯、总胆固醇、瘦素和脂联素的含量。制作脂肪组织石蜡切片,经苏木精-伊红(HE)染色颜色观察脂肪细胞的变化。取附睾脂肪经实时荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,q RT-PCR),观察小鼠体内肥胖基因(瘦素和脂联素基因)m RNA表达变化。结果:Hx干预组小鼠的脂肪质量显著低于模型组(P<0.05);总胆固醇含量水平均显著低于模型组(P<0.05、P<0.01);Hx中、低剂量组和对照组的瘦素和脂联素m RNA表达量显著低于模型组(P<0.05)。结论:Hx中、低剂量组有较好的抗肥胖作用,其中低剂量组的抗肥胖效果最好。小鼠脂肪细胞切片中脂肪组织形态学为小鼠肥胖造模提供进一步的研究依据。

Objective： To explore the anti-obesity effect of hypoxanthine （Hx） on nutritionally obese mice. Methods：An obese mouse model was established successfully by feeding high-fat diet. The ICR mice were randomly dividedinto six groups, namely, normal control, model control, positive control （L-carnitine, 100 mg/kg） and hypoxanthinetreatment groups （30, 60, and 90 mg/kg）. After 4 weeks of oral administration, blood samples were collected andcentrifuged and then the mice were sacrificed. The contents of triglyceride, total cholesterol, leptin and adiponectinin mouse serum were detected. The adipose tissues were made into paraffin section to observe the changes of adiposecells by HE staining. The gene expression levels of leptin and adiponectin in mouse epididymal adipose tissuewere measured by real-time quantitative polymerase chain reaction （qRT-PCR）. Results： The body weight of theHx treatment groups were significantly lower than that of the model group （P 〈 0.05）; TC levels were significantlylower than those of the model group （P 〈 0.05, P 〈 0.01）. The gene expression levels of leptin and adiponectin inthe middle- and low-dose Hx groups and normal control were significantly different from those of the model group（P 〈 0.05）. Conclusion： The middle- and low-dose Hx groups had good anti-obesity effect, and the best effect was achievedat the low dose.