应用Illumina MiSeq高通量测序技术解析O_3-BAC饮用水处理过程细菌多样性变化

Analysis of Bacterial Diversity in O_3-BAC Drinking Water Treatment Process by Using Illumina MiSeq High Throughput Sequencing Technology

采用Illumina MiSeq高通量测序技术,对臭氧-生物活性炭水处理工艺过程各单元出水中细菌多样性及丰度进行研究,测序获得196 809条16S r DNA基因序列,归类为38个门,522个属。各样品中细菌多样性分析结果表明:在各处理单元出水中细菌群落具有高度多样性,预臭氧和臭氧氧化处理对水体中细菌多样性及丰度的影响最大,可杀灭一部分属的细菌,可合理控制臭氧浓度杀灭部分耐氯菌;絮凝沉淀和沙滤单元处理对水体中细菌多样性具有恢复效果,使水体中细菌种属进一步增多;在生物活性炭滤池处理后,细菌多样性增加,丰度分布更为均匀,对后期消毒工艺提出了更高的要求。

Illumina MiSeq high-throughput sequencing technology was used to study the bacterial diversity and abundanceof the effluent water from each unit process in O3-BAC water treatment. A total of 196 809 16S rDNA sequences wereacquired, which were classified as 522 genera in 38 phyla. The results showed that there was high bacterial communitydiversity in the processing unit effluent. Pre-ozonation and ozone oxidation treatment of water influenced most significantlybacterial diversity and abundance. Ozone could kill some bacteria which were hard to be killed by chlorine disinfection ina reasonable ozone concentration range. Flocculation and sand filtration could recover the bacterial diversity in water, asdemonstrated number of bacterial species. In biological activated carbon filter treatment, bacterial diversity was increasedand their abundance distribution was more uniform, which would propose higher requirements for later disinfection technics.