HPLC梯度洗脱法同时测定小儿暑感宁糖浆中的香荆芥酚、麝香草酚、和厚朴酚及厚朴酚

Simultaneous determination of carvacrol, thymol, honokiol and magnolol in Xiao'er shuganning syrup by HPLC with gradient elution

目的:建立用高效液相色谱法(HPLC)同时测定小儿暑感宁糖浆中的香荆芥酚、麝香草酚、和厚朴酚及厚朴酚含量的分析方法。方法:采用HPLC法,色谱柱:Eclipse XDB-C_(18)柱(250 mm×4.6 mm,5μm);流动相为甲醇(A)-0.05%冰醋酸溶液(B),梯度洗脱;柱温:30℃;流速:1.0 m L·min^(-1);进样量:20μL;香荆芥酚、麝香草酚检测波长为λ_1=274 nm,和厚朴酚、厚朴酚检测波长为λ_2=294 nm。结果:香荆芥酚、麝香草酚、和厚朴酚及厚朴酚线性范围分别在6.35~127.00μg·m L^(-1)(r=0.999 3)、4.74~94.80μg·m L^(-1)(r=0.999 1)、4.35~87.00μg·m L^(-1)(r=0.999 7)、5.19~103.80μg·m L^(-1)(r=0.999 4)范围内线性关系良好。精密度、重现性实验中各组分RSD值均小于2.0%;4种成分的平均加样回收率(n=9)及RSD分别为98.18%(1.15%)、96.94%(1.44%)、99.03%(0.92%)、97.24%(0.79%)。结论:本文建立的HPLC法简便、准确、重复性好,可同时测定小儿暑感宁糖浆中的香荆芥酚、麝香草酚、和厚朴酚及厚朴酚含量,为该制剂的质量控制提供理论参考。

Objective： To establish the HPLC method for simultaneous determination of carvacrol, thymol, honokiol and magnolol in Xiao＇er shuganning syrup. Methods： The Eclipse XDB-C18 column（250 mm × 4.6 mm, 5 μm） was used, with a mobile phase consisted of methanol（mobile phase A） and 0.05% glacial acetic acid solution（mobile phase B） for gradient elution. The column temperature was 30 ℃. The flow rate was 1.0 m L·min^-1. The injection volume was 20 μL. Carvacrol and thymol were detected at 274 nm, honokiol and magnolol were detected at 294 nm. Results： Carvacrol, thymol, honokiol and magnolol had good linearities at 6.35 – 127.00 μg·m L^-1（r = 0.999 3）, 4.74 – 94.80 μg·m L^-1（r = 0.999 1）, 4.35 – 87.00 μg·m L^-1（r = 0.999 7）, 5.19 – 103.80 μg·m L^-1（r = 0.999 4）, respectively. In precision and reproducibility test, the RSD results of 4 components were less than 2.0%; the average recovery and their RSD results were 98.18%（1.15%）, 96.94%（1.44%）, 99.03%（0.92%）, 97.24%（0.79%）, respectively. Conclusion： The HPLC method is simple and reliable with a good repeatability, which can simultaneously determine the contents of carvacrol, thymol, honokiol and magnolol in Xiao＇er shuganning syrup. This method can be used to evaluate the quality of this preparation.