猪、鸡源分离粪肠球菌的核糖体分型及耐药性分析

Analysis of Ribosome Genotyping and Antimicrobial Resistance in Enterococcus faecalis Isolated from Pigs and Chickens

对上海地区1个规模化养猪场和1个规模化养鸡场分离鉴定的40株粪肠球菌,采用微量肉汤稀释法对12种抗菌药物进行药敏试验,采用Riboprinter?微生物基因指纹鉴定系统检测分离菌株的基因指纹图谱,分别检测分离菌株中的核糖体型和耐药性。结果表明,在40株粪肠球菌中,80%以上为多重耐药菌株,有4株猪源粪肠球菌对万古霉素耐药,其中有2株表现为对万古霉素高度耐药(MIC>64mg/L),且对链霉素和庆大霉素同时表现为高度耐药(MIC>2 048mg/L)。从耐药表型和核糖体分型共同分析粪肠球菌的耐药性,发现多重耐药性严重,同一核糖体型菌株的耐药表型并非完全一致。

This study was to investigate the ribosome genotyping and antimicrobial resistance in Enterococcus faecalis strains isolated from 2large-scale farms in Shanghai.40 isolates were evaluated for their sensitivity to 12 antimicrobial agents by broth microdilution,and the ribosome genotyping（ribotype）was characterized by the Riboprinter？Microbial Characterization System.The resistance rates of most antimicrobial drug were relatively high and multidrug resistant strains were detected with more than 80%.4strains of Enterococcus faecalis isolated from pigs were resistant to vancomycin,including 2strains of vancomycin highly resistant（MIC64mg/L）with the same time highly resistant to gentamicin and streptomycin（MIC2 048mg/L）.The results showed that multidrug resistance of Enterococcus faecalis was a serious issue,and the resistant phenotypes of the same type of ribotype was not entirely consistent.