一种芦荟成分为主保健食品通便功效的动物试验研究

Experimental animal study on the effect of aloe leave as the main component of the health food on catharsis

目的评价以芦荟全叶冻干粉、小麦膳食纤维、苹果醋粉等为主要成分的保健食品通便功能。方法按卫生部《保健食品检验与评价技术规范》(2003年版)中润肠通便功能检验方法开展实验。经口给予小鼠3.0、1.0、0.5 g/kg体质量(相当人体推荐量的30、10、5倍)剂量的受试物15 d,采用不同剂量的复方地芬诺酯制备肠蠕动抑制模型和便秘模型。测定肠蠕动抑制模型小鼠小肠墨汁推进率、便秘小鼠排便时间、排粪便粒数和粪便重量。结果各剂量组小鼠各周体质量、体质量增加值未见明显变化;受试物低、中剂量组的小肠推进速度、第一粒黑便排出时间、6 h排黑便粒数和黑便总重量与模型对照组比较变化不明显,差异无统计学意义(P>0.05);受试物高剂量组的小肠墨汁推进率和墨汁推进转换值则有明显增加,第一粒黑便排出时间提前,6 h排黑便粒数和黑便总重量有明显增加,与模型对照组比较,统计学分析差异有统计学意义(P<0.05)。结论根据《保健食品检验与评价技术规范》(2003年版),该受试物具有通便功能。

Objective To evaluate the effect of aloe leave freeze-dried powder, wheat food fiber and Pingguocu powder as the main ingredients of health food on catharsis.Methods Experiments were carried out in accordance with the detection methods for loo-sing the bowel to relieve constipation specified in＂the Health Food Inspection and Evaluation Technical Specifications＂of the Ministry of Health （2003 Edition） .In animal experiments, the KM mice were given the test substance at a dosage of 3.0, 1.0 and 0.5 g /kg BW （ roughly equal to 10, 30 and 5 times the recommended amount for humans） for a succession of 15 days.Then, the intestinal peri-stalsis inhibition model and constipation model were developed by using different doses of compound diphenoxylate.The driving rate of feces, defecation time, the amount of defecation and the total weight of defecation were determined in the mice of the intestinal peristal-sis inhibition model.Results The KM mice were given the test substance by gavage at dosages of 3.0g, 1, 0g and 0.5g kg BW, for re-spectively 7 and 15 days.For the animals given different doses, no obvious changes could be noted in the body weight and rate of weight gain.Significant changes could neither be seen in intestinal driving rate, the defecation time of the first grain of feces, total grains of black feces in 6 hours and total weight of black feces for the low-dose and medium-dose animals, and there was no statistical signifi-cance, when comparisons were made between them（P〉0.05）.The intestinal feces driving rate and feces driving transfer rate of the high-dose animals increased significantly.The first grain of black feces was ahead of time, the total grains of black feces within 6 hours and total discharge of black feces also increased significantly.Significant differences could be noticed, when it was compared with that of the model control group（P〈0.05）.Conclusion In accordance with the ＂Technical Specifications for Health Food Inspection and Evaluation＂（2003 Edition）, this testing substance seemed to have the function of defecation.